Habibi Alireza, Pakpour Roudsari Masoumeh, ZiaZiabari Seyyed Mahdi
Department of Basic Sciences, Faculty of Sciences, Imam Hossein University, Tehran, Iran.
Department of Education and Training, Roudsar, Iran.
AMB Express. 2025 Jul 15;15(1):107. doi: 10.1186/s13568-025-01915-4.
The research investigated the effects of that magnetic nanoparticles coated with the plant-based flavonoid quercetin have on the growth of Staphylococcus aureus. The synthesis of magnetic nanoparticles proceeded through co-precipitation and further involved quercetin coating using a dextran stabilizer. A combined product of these materials received the chemical name FeO@Dex-QT. The study included multiple analytical tests such as FTIR (Fourier Transform Infrared Spectroscopy), FE-SEM (Field Emission Scanning Electron Microscopy), EDS (Energy-Dispersive X-Ray Spectroscopy) and XRD (X-ray diffraction) together with cellular and molecular assays. Biofilm detection experiments showed that 95% of studied microorganisms had moderate-to-strong biofilm formation abilities. The examined strains that formed biofilms exhibited complete biofilm inhibition at a minimum concentration of 512 μg/mL. Post-treatment with FeO@Dex-QT nanoparticles, the Coa and Hla genes were expressed at 30 and 20% levels respectively, as determined through RT-PCR analysis. The research uncovered that FeO@Dex-QT nanoparticles demonstrate successful antibacterial properties against S. aureus strains, making them viable for substituting conventional antibiotics.
该研究调查了包覆有植物类黄酮槲皮素的磁性纳米颗粒对金黄色葡萄球菌生长的影响。磁性纳米颗粒的合成通过共沉淀法进行，并且进一步使用葡聚糖稳定剂进行槲皮素包覆。这些材料的组合产物的化学名称为FeO@Dex-QT。该研究包括多种分析测试，如傅里叶变换红外光谱（FTIR）、场发射扫描电子显微镜（FE-SEM）、能量色散X射线光谱（EDS）和X射线衍射（XRD），以及细胞和分子分析。生物膜检测实验表明，95%的受试微生物具有中度至强的生物膜形成能力。形成生物膜的受试菌株在最低浓度为512μg/mL时表现出完全的生物膜抑制。通过逆转录聚合酶链反应（RT-PCR）分析确定，用FeO@Dex-QT纳米颗粒处理后，Coa和Hla基因的表达水平分别为30%和20%。该研究发现，FeO@Dex-QT纳米颗粒对金黄色葡萄球菌菌株具有成功的抗菌特性，使其有望替代传统抗生素。
