Microbiology Service, University Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain.
Microbiology Service, University Hospital Marqués de Valdecilla-IDIVAL, Santander, Spain.
Enferm Infecc Microbiol Clin (Engl Ed). 2023 Feb;41(2):99-102. doi: 10.1016/j.eimce.2022.11.016.
The correct identification of the species within the Candida parapsilosis complex has become relevant due to the resistance of Candida metapsilosis to antifungals. We describe the characteristics of the Candida parapsilosis complex isolates, with respect to antifungal resistance and biofilm formation.
We perform a descriptive cross-sectional study in 30 strains, collected in a tertiary hospital. All strains, were identified by Vitek2, Vitek-MS™ systems and by ITS sequencing. The antifungal susceptibility profile was obtained with Sensititre™ panels, while biomass production and metabolic activity were quantified by means of crystal violet and XTT reduction assay, respectively.
There was a 100% correlation between Vitek-MS™ and ITS sequencing. All isolates were susceptible to the nine antifungals tested. The metabolic activity and biomass production tests did not show any difference among the subtypes.
The Vitek-MS™ system provides acceptable identification. We did not find significant differences neither in azole resistance nor in biofilm formation.
由于 Candida metapsilosis 对抗真菌药物的耐药性，正确鉴定近平滑念珠菌复合体中的物种变得尤为重要。我们描述了念珠菌假丝酵母复合体分离株的特征，包括抗真菌耐药性和生物膜形成。
我们在一家三级医院进行了一项描述性的横断面研究，共收集了 30 株菌株。所有菌株均通过 Vitek2、Vitek-MS™系统和 ITS 测序进行鉴定。使用 Sensititre™ 试剂盒获得抗真菌药敏谱，通过结晶紫和 XTT 还原测定分别定量生物量产生和代谢活性。
Vitek-MS™ 和 ITS 测序之间存在 100%的相关性。所有分离株均对测试的九种抗真菌药物敏感。代谢活性和生物量产生试验在各亚型之间没有显示出任何差异。
Vitek-MS™ 系统提供了可接受的鉴定。我们没有发现唑类耐药性或生物膜形成方面的显著差异。
