Kawicha Praphat, Nitayaros Jariya, Saman Prakob, Thaporn Sirikanya, Thanyasiriwat Thanwanit, Somtrakoon Khanitta, Sangdee Kusavadee, Sangdee Aphidech
Plant Pest and Biocontrol Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand.
Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand.
Plant Pathol J. 2023 Feb;39(1):108-122. doi: 10.5423/PPJ.OA.08.2022.0124. Epub 2023 Feb 1.
Fusarium oxysporum f. sp. lycopersici (Fol) and Fusarium oxysporum f. sp. cubense (Foc), are the causal agent of Fusarium wilt disease of tomato and banana, respectively, and cause significant yield losses worldwide. A cost-effective measure, such as biological control agents, was used as an alternative method to control these pathogens. Therefore, in this study, six isolates of the Streptomyces-like colony were isolated from soils and their antagonistic activity against phytopathogenic fungi and plant growth-promoting (PGP) activity were assessed. The results showed that these isolates could inhibit the mycelial growth of Fol and Foc. Among them, isolate STRM304 showed the highest percentage of mycelial growth reduction and broad-spectrum antagonistic activity against all tested fungi. In the pot experiment study, the culture filtrate of isolates STRM103 and STRM104 significantly decreased disease severity and symptoms in Fol inoculated plants. Similarly, the culture filtrate of the STRM304 isolate significantly reduced the severity of the disease and symptoms of the disease in Foc inoculated plants. The PGP activity test presents PGP activities, such as indole acetic acid production, phosphate solubilization, starch hydrolysis, lignin hydrolysis, and cellulase activity. Interestingly, the application of the culture filtrate from all isolates increased the percentage of tomato seed germination and stimulated the growth of tomato plants and banana seedlings, increasing the elongation of the shoot and the root and shoot and root weight compared to the control treatment. Therefore, the isolate STRM103 and STRM104, and STRM304 could be used as biocontrol and PGP agents for tomato and banana, respectively, in sustainable agriculture.
尖孢镰刀菌番茄专化型（Fol）和尖孢镰刀菌香蕉专化型（Foc）分别是番茄和香蕉枯萎病的病原菌，在全球范围内造成重大产量损失。一种具有成本效益的措施，如生物防治剂，被用作控制这些病原菌的替代方法。因此，在本研究中，从土壤中分离出6株类链霉菌菌落，并评估了它们对植物病原真菌的拮抗活性和植物生长促进（PGP）活性。结果表明，这些分离株能够抑制Fol和Foc的菌丝生长。其中，分离株STRM304对所有测试真菌的菌丝生长抑制率最高，且具有广谱拮抗活性。在盆栽试验研究中，分离株STRM103和STRM104的培养滤液显著降低了接种Fol的植株的病情严重程度和症状。同样，分离株STRM304的培养滤液显著降低了接种Foc的植株的病情严重程度和症状。PGP活性测试显示了PGP活性，如吲哚乙酸产生、磷溶解、淀粉水解、木质素水解和纤维素酶活性。有趣的是，所有分离株的培养滤液的应用提高了番茄种子的发芽率，刺激了番茄植株和香蕉幼苗的生长，与对照处理相比，增加了茎和根的伸长以及茎和根的重量。因此，分离株STRM103、STRM104和STRM304可分别用作可持续农业中番茄和香蕉的生物防治剂和PGP剂。
