Chen Jie, Guo Weichang, Yin Hejia, Ma Liju, Li Shaoyou, Li Huifang
Department of Diabetes, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, People's Republic of China.
Department of Physical Education, Kunming Medical University, Kunming, 650032, People's Republic of China.
Diabetes Metab Syndr Obes. 2023 Jan 24;16:213-223. doi: 10.2147/DMSO.S398264. eCollection 2023.
This study aimed to explore relationship among RANTES -28 (rs2280788) C/G polymorphism or CCR5 59029 (rs1799987) A/G polymorphism, level of self-expression, and type 2 diabetes mellitus (T2DM).
Clinical data were collected from 92 subjects with normal blood glucose (NC) and 97 patients with T2DM (DM). CCR5 levels on the surface of monocyte/lymphocyte and plasma RANTES levels were detected by flow cytometry. TaqMan real-time fluorescent quantitative PCR was used to detect genetic polymorphisms of RANTES rs2280788 and CCR5 rs1799987.
There were no significant differences in frequencies of CCR5 rs1799987 genotype and A/G allele and frequencies of RANTES rs2280788 genotype and C/G allele, between subjects in NC and DM group ( > 0.05). Plasma RANTES level in DM group was significantly lower than NC group ( < 0.05), and difference came from patients with T2DM using insulin and subjects with normal blood glucose. CCR5 levels on the surface of monocytes and lymphocytes of patients in DM group were higher than NC group ( < 0.05). There was no significant difference in CCR5 level on the surface of monocytes and lymphocytes (or plasma RANTES level) among different genotypes of CCR5 rs1799987 (or RANTES rs2280788) ( > 0.05). RANTES level was positively correlated with age and TC and negatively correlated with diabetes course and HbA1c. CCR5 level on the surface of monocytes was positively correlated with drinking years, HbA1c, course of diabetes, and negatively correlated with TC. CCR5 on lymphocyte surface was positively correlated with diabetes course, smoking years, HbA1c, and negatively correlated with LDL, TC, HDL ( < 0.05).
RANTES -28 (rs2280788) C/G polymorphism or CCR5 59029 (rs1799987) A/G polymorphism may not be associated with T2DM of Han nationality in Kunming and cannot affect RANTES and CCR5 expression. RANTES and CCR5 levels may be related to T2DM but may also be affected by age, blood lipids, HbA1c, diabetes course, drugs, and other factors.
本研究旨在探讨RANTES -28（rs2280788）C/G多态性或CCR5 59029（rs1799987）A/G多态性、自我表达水平与2型糖尿病（T2DM）之间的关系。
收集92例血糖正常（NC）受试者和97例T2DM患者（DM）的临床资料。采用流式细胞术检测单核细胞/淋巴细胞表面CCR5水平和血浆RANTES水平。应用TaqMan实时荧光定量PCR检测RANTES rs2280788和CCR5 rs1799987的基因多态性。
NC组和DM组受试者的CCR5 rs1799987基因型和A/G等位基因频率以及RANTES rs2280788基因型和C/G等位基因频率无显著差异（>0.05）。DM组血浆RANTES水平显著低于NC组（<0.05），差异来自使用胰岛素的T2DM患者和血糖正常受试者。DM组患者单核细胞和淋巴细胞表面CCR5水平高于NC组（<0.05）。CCR5 rs1799987（或RANTES rs2280788）不同基因型的单核细胞和淋巴细胞表面CCR5水平（或血浆RANTES水平）无显著差异（>0.05）。RANTES水平与年龄和总胆固醇呈正相关，与糖尿病病程和糖化血红蛋白呈负相关。单核细胞表面CCR5水平与饮酒年限、糖化血红蛋白、糖尿病病程呈正相关，与总胆固醇呈负相关。淋巴细胞表面CCR5与糖尿病病程、吸烟年限、糖化血红蛋白呈正相关，与低密度脂蛋白、总胆固醇、高密度脂蛋白呈负相关（<0.05）。
RANTES -28（rs2280788）C/G多态性或CCR5 59029（rs1799987）A/G多态性可能与昆明汉族T2DM无关，且不影响RANTES和CCR5表达。RANTES和CCR5水平可能与T2DM有关，但也可能受年龄、血脂、糖化血红蛋白、糖尿病病程、药物等因素影响。
