Chen Song, Liu Changhua, Hao Yuanmin, Liu Yueyue, Liu Xu, Du Chao
Key Laboratory of Integrated Crop Pest Management in Southwest China (Ministry of Agriculture), Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu, China Institute of Plant Protection, Sichuan Academy of Agricultural Sciences Chengdu China.
Institute of Quality Standard and Testing Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu, China Institute of Quality Standard and Testing Technology Research, Sichuan Academy of Agricultural Sciences Chengdu China.
Zookeys. 2022 Jul 1;1109:103-114. doi: 10.3897/zookeys.1109.81544. eCollection 2022.
Oil beetles are meloids, which are characterised for their cleptoparasitic habits in bee nests and oily fluid of cantharidin that causes blistering and swelling of the skin. The complete mitochondrial genome of is determined using the next-generation sequencing technology and its genomic characteristics are described. The 15,653-bp long genome is a circular molecule consisting of 13 protein-coding genes (PCG), 22 transport RNA, two ribosomal RNA, and a control region. The A + T bias of the mitochondrial genome is manifested in the complete sequence and the codon usage of protein-coding genes. The genetic distance within and between genera is calculated to confirm the taxonomic status of . The phylogenetic relationships among 15 available meloid taxa are inferred by the maximum likelihood (ML) method based on 13 mitochondrial PCGs. The ML trees resulting from nucleotide and amino acid datasets recover both the monophyly of and and the polyphyly comprising and . This study provides the first description of a mitochondrial genome belonging to the genus . The mitochondrial genome sequence and its characteristics are expected to be conducive to future studies on taxonomy, systematics, and molecular phylogenetics of the family Meloidae.
芫菁科甲虫属于芫菁类，其特点是在蜂巢中有盗寄生习性，且含有导致皮肤起泡和肿胀的斑蝥素油性液体。利用下一代测序技术测定了[物种名称]的完整线粒体基因组，并描述了其基因组特征。这个长度为15,653 bp的基因组是一个环状分子，由13个蛋白质编码基因（PCG）、22个转运RNA、两个核糖体RNA和一个控制区组成。线粒体基因组的A + T偏向性体现在完整序列和蛋白质编码基因的密码子使用上。计算属内和属间的遗传距离以确认[物种名称]的分类地位。基于13个线粒体PCG，通过最大似然法（ML）推断15个可用芫菁类分类单元之间的系统发育关系。由核苷酸和氨基酸数据集生成的ML树既恢复了[属名1]和[属名2]的单系性，也恢复了包含[属名3]和[属名4]的多系性。本研究首次描述了属于[属名]的线粒体基因组。线粒体基因组序列及其特征有望有助于芫菁科未来在分类学、系统学和分子系统发育学方面的研究。
