Institute of Entomology and Special Key Laboratory for Development and Utilization of Insect Resources of Guizhou, Guizhou University, Guiyang 550025, China.
J Insect Sci. 2021 Nov 1;21(6). doi: 10.1093/jisesa/ieab100.
The mitochondrial genome sequences of Denierella emmerichi, Epicauta curvispina, and Meloe poggii were determined. Their mitochondrial genomes were found to contain 37 genes (13 protein-coding genes [PCGs], 22 tRNA genes, and 2 rRNAs), of which 4 PCGs, 8 tRNA genes, and 2 rRNAs are encoded by the N-strand, and the remaining genes are encoded by the J-strand. The mitochondrial genomes of D. emmerichi, E. curvispina, and M. poggii are 15,702 bp, 15,813 bp, and 15,626 bp in length, respectively, and their guanine-cytosine contents are 28%, 33%, and 36%, respectively. The 13 PCGs of D. emmerichi, E. curvispina, and M. poggii use ATN as the standard start codon and TAA, TAG, and T as the stop codons. The Bayesian inference and maximum likelihood phylogenetic analysis results based on the 13 PCGs and 13 PCGs + 2rRNAs datasets of the mitochondrial genomes of the Meloidae support Epicauta (Coleoptera: Meloidae) ([D. emmerichi, E. curvispina, E. ruficeps, E. aptera] + [E. chinensis, E. impressicornis, E. gorhami, E. tibialis]). We believe that this research enriches the literature on the mitochondrial genomics of Meloidae and serves as a foundation for the further study of the phylogenetic relationships and characterization of Meloidae and Coleoptera.
测定了德内雷拉·埃梅里希、弯曲金龟和 Meloe poggii 的线粒体基因组序列。发现它们的线粒体基因组包含 37 个基因（13 个蛋白质编码基因[PCGs]、22 个 tRNA 基因和 2 个 rRNA），其中 4 个 PCGs、8 个 tRNA 基因和 2 个 rRNA 由 N 链编码，其余基因由 J 链编码。D. emmerichi、E. curvispina 和 M. poggii 的线粒体基因组分别长 15702bp、15813bp 和 15626bp，G+C 含量分别为 28%、33%和 36%。D. emmerichi、E. curvispina 和 M. poggii 的 13 个 PCGs 使用 ATN 作为标准起始密码子，TAA、TAG 和 T 作为终止密码子。基于线粒体基因组的 13 个 PCGs 和 13 个 PCGs+2rRNAs 数据集的贝叶斯推断和最大似然系统发育分析结果支持 Epicauta（鞘翅目：金龟子科）[D. emmerichi、E. curvispina、E. ruficeps、E. aptera+[E. chinensis、E. impressicornis、E. gorhami、E. tibialis]。我们相信这项研究丰富了金龟子科线粒体基因组学的文献，并为进一步研究金龟子科和鞘翅目昆虫的系统发育关系和特征提供了基础。
