Tong Yao, Guan Bingxin, Sun Zhiwei, Dong Xiangjun, Chen Yuqing, Li Yanru, Jiang Yanyan, Li Juan
Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong, China.
Department of Pathology, The Second Hospital of Shandong University, Jinan, Shandong, China.
Talanta. 2023 May 15;257:124307. doi: 10.1016/j.talanta.2023.124307. Epub 2023 Feb 4.
piR-823 is a newly discovered colorectal cancer marker with high diagnostic efficacy. However, the current quantification methods have complicated operations and high cost, which restrict its clinical application. Herein, a metal-organic framework (MOF) with a UiO-66 prototype structure which supports gold nanoclusters (Au NCs), Au NCs/UiO-66-NH, were prepared as a model nanobiosensing platform for ratiometric detection of exosomal piR-823. The rolling circle amplification process provides high sensitivity and the ratiometric detection process ensures good accuracy of the sensor. Such biosensor showed a wide linear range of 0.04-4 pM, and a low detection limit of 10.2 fM towards piR-823. In addition, piR-823 can be used as an effective supplement to carcinoembryonic antigen (CEA) in clinical diagnosis of colorectal cancer. This study not only provides a potentially valuable ratio fluorescence platform involving enzyme catalytic reaction, but also offers a design blueprint for further expansion of nanotechnology in the diverse biological analysis.
piR - 823是一种新发现的具有高诊断效能的结直肠癌标志物。然而，目前的定量方法操作复杂且成本高昂，限制了其临床应用。在此，制备了一种具有UiO - 66原型结构并负载金纳米簇（Au NCs）的金属有机框架（MOF），即Au NCs/UiO - 66 - NH₂，作为用于外泌体piR - 823比率检测的模型纳米生物传感平台。滚环扩增过程提供了高灵敏度，比率检测过程确保了传感器的良好准确性。这种生物传感器对piR - 823显示出0.04 - 4 pM的宽线性范围和10.2 fM的低检测限。此外，在结直肠癌的临床诊断中，piR - 823可作为癌胚抗原（CEA）的有效补充。本研究不仅提供了一个涉及酶催化反应的潜在有价值的比率荧光平台，还为纳米技术在各种生物分析中的进一步扩展提供了设计蓝图。
