扫描量热法揭示了L-α-二棕榈酰磷脂酰胆碱中的一种新的相变。
Scanning calorimetry reveals a new phase transition in L-alpha-dipalmitoylphosphatidylcholine.
作者信息
Slater J L, Huang C
机构信息
Department of Biochemistry, University of Virginia School of Medicine, Charlottesville 22908.
出版信息
Biophys J. 1987 Oct;52(4):667-70. doi: 10.1016/S0006-3495(87)83260-5.
Abstract
We report a new phase transition in fully hydrated dispersions of dipalmitoylphosphatidylcholine (DPPC). This new transition, called the sub-subtransition, exhibits a transition enthalpy of 0.25 kcal/mol with a Tm at 6.8 degrees C. Unlike the subtransition, no extended low temperature incubation is required to observe the sub-subtransition. This new sub-subgel (SGII) phase may be a precursor to the subgel (SGI) phase, and this discovery is discussed in relation to the current knowledge regarding the polymorphic gel phases of both ester- and ether-linked lipids with identical acyl chains.
摘要
我们报道了二棕榈酰磷脂酰胆碱（DPPC）完全水合分散体中的一种新的相变。这种新的转变，称为亚亚转变，其转变焓为0.25千卡/摩尔，熔点为6.8摄氏度。与亚转变不同，观察亚亚转变不需要长时间的低温孵育。这种新的亚亚凝胶（SGII）相可能是亚凝胶（SGI）相的前体，并且结合目前关于具有相同酰基链的酯键和醚键连接脂质的多晶型凝胶相的知识对这一发现进行了讨论。
相似文献
1
Scanning calorimetry reveals a new phase transition in L-alpha-dipalmitoylphosphatidylcholine.扫描量热法揭示了L-α-二棕榈酰磷脂酰胆碱中的一种新的相变。
Biophys J. 1987 Oct;52(4):667-70. doi: 10.1016/S0006-3495(87)83260-5.
2
The interfacial structure of phospholipid bilayers: differential scanning calorimetry and Fourier transform infrared spectroscopic studies of 1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine and its dialkyl and acyl-alkyl analogs.磷脂双层的界面结构：1,2-二棕榈酰-sn-甘油-3-磷酸胆碱及其二烷基和酰基-烷基类似物的差示扫描量热法和傅里叶变换红外光谱研究
Biophys J. 1996 Jun;70(6):2736-46. doi: 10.1016/S0006-3495(96)79843-0.
3
Rapid reversible formation of a metastable subgel phase in saturated diacylphosphatidylcholines.饱和二酰基磷脂酰胆碱中亚稳态亚凝胶相的快速可逆形成。
Biophys J. 1995 Jun;68(6):2370-5. doi: 10.1016/S0006-3495(95)80419-4.
4
Kinetics for the subgel phase formation in DPPC/DOPC mixed bilayers.在 DPPC/DOPC 混合双层中亚凝胶相形成的动力学。
Chem Phys Lipids. 2010 Sep;163(7):712-9. doi: 10.1016/j.chemphyslip.2010.06.007. Epub 2010 Jul 1.
5
Subgel studies of dimyristoylphosphatidylcholine bilayers.二肉豆蔻酰磷脂酰胆碱双层膜的亚凝胶研究。
J Phys Chem B. 2006 Nov 9;110(44):22192-6. doi: 10.1021/jp055178s.
6
Bilayer interactions of ether- and ester-linked phospholipids: dihexadecyl- and dipalmitoylphosphatidylcholines.醚键连接和酯键连接的磷脂的双层相互作用：二己基磷脂酰胆碱和二棕榈酰磷脂酰胆碱。
Biochemistry. 1987 Oct 20;26(21):6599-603. doi: 10.1021/bi00395a006.
7
A DSC and FTIR spectroscopic study of the effects of the epimeric 4-cholesten-3-ols and 4-cholesten-3-one on the thermotropic phase behaviour and organization of dipalmitoylphosphatidylcholine bilayer membranes: comparison with their 5-cholesten analogues.DSC 和 FTIR 光谱研究差向异构体 4-胆甾烯-3-醇和 4-胆甾烯-3-酮对二棕榈酰磷脂酰胆碱双层膜热致相态行为和组织的影响：与 5-胆甾烯类似物的比较。
Chem Phys Lipids. 2014 Jan;177:71-90. doi: 10.1016/j.chemphyslip.2013.11.008. Epub 2013 Dec 1.
8
Subgel-phase formation of membranes of ether-linked phosphatidylcholines.醚键连接的磷脂酰胆碱的亚凝胶相形成。
Chem Phys Lipids. 2021 Sep;239:105119. doi: 10.1016/j.chemphyslip.2021.105119. Epub 2021 Jul 21.
9
Kinetics of the subtransition in dipalmitoylphosphatidylcholine.二棕榈酰磷脂酰胆碱中亚转变的动力学
Biochemistry. 1987 Jul 14;26(14):4288-94. doi: 10.1021/bi00388a016.
10
New aspects of the interaction of cholesterol with dipalmitoylphosphatidylcholine bilayers as revealed by high-sensitivity differential scanning calorimetry.高灵敏度差示扫描量热法揭示的胆固醇与二棕榈酰磷脂酰胆碱双层膜相互作用的新方面
Biochim Biophys Acta. 1995 Mar 8;1234(1):90-8. doi: 10.1016/0005-2736(94)00266-r.
引用本文的文献
1
Pressure perturbation and differential scanning calorimetric studies of bipolar tetraether liposomes derived from the thermoacidophilic archaeon Sulfolobus acidocaldarius.源自嗜热嗜酸古菌嗜酸热硫化叶菌的双极四醚脂质体的压力扰动和差示扫描量热研究。
Biophys J. 2005 Sep;89(3):1841-9. doi: 10.1529/biophysj.105.063933. Epub 2005 Jun 24.
2
New ordered metastable phases between the gel and subgel phases in hydrated phospholipids.水合磷脂中凝胶相和亚凝胶相之间新的有序亚稳相。
Biophys J. 2001 Apr;80(4):1873-90. doi: 10.1016/S0006-3495(01)76157-7.
3
Rapid reversible formation of a metastable subgel phase in saturated diacylphosphatidylcholines.饱和二酰基磷脂酰胆碱中亚稳态亚凝胶相的快速可逆形成。
Biophys J. 1995 Jun;68(6):2370-5. doi: 10.1016/S0006-3495(95)80419-4.
本文引用的文献
1
Pressure locking of the subgel phase of hydrated dipalmitoyl phosphatidylcholine bilayers: a Raman spectroscopic study.水合二棕榈酰磷脂酰胆碱双层亚凝胶相的压力锁定：拉曼光谱研究。
Biophys J. 1986 Jun;49(6):1199-203. doi: 10.1016/S0006-3495(86)83748-1.
2
Scanning calorimetric evidence for a third phase transition in phosphatidylcholine bilayers.扫描量热法证明磷脂酰胆碱双层膜中存在第三次相变。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5060-3. doi: 10.1073/pnas.77.9.5060.
3
Dilatometric studies of the subtransition in dipalmitoylphosphatidylcholine.二棕榈酰磷脂酰胆碱亚转变的膨胀计研究。
Biochemistry. 1982 Aug 3;21(16):3817-21. doi: 10.1021/bi00259a015.
4
Characterization of a third phase transition in multilamellar dipalmitoyllecithin liposomes.多层二棕榈酰卵磷脂脂质体中第三种相变的表征
Biochemistry. 1981 Sep 29;20(20):5707-10. doi: 10.1021/bi00523a011.
5
Interdigitated hydrocarbon chain packing causes the biphasic transition behavior in lipid/alcohol suspensions.相互交错的烃链堆积导致脂质/醇悬浮液中出现双相转变行为。
Biochim Biophys Acta. 1984 Jun 13;773(1):169-72. doi: 10.1016/0005-2736(84)90562-5.
6
A low-temperature structural phase transition of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers in the gel phase.凝胶相中1,2-二棕榈酰-sn-甘油-3-磷酸胆碱双层膜的低温结构相变
Biochim Biophys Acta. 1983 Jul 13;732(1):92-8. doi: 10.1016/0005-2736(83)90190-6.
7
Phosphatidylcholine bilayers: subtransitions in pure and in mixed lipids.磷脂酰胆碱双层膜：纯脂质和混合脂质中的亚转变
Chem Phys Lipids. 1984 Aug;35(3):291-7. doi: 10.1016/0009-3084(84)90053-7.
8
Raman spectroscopy of thermotropic and high-pressure phases of aqueous phospholipid dispersions.水性磷脂分散体的热致相和高压相的拉曼光谱
Annu Rev Biophys Bioeng. 1984;13:1-24. doi: 10.1146/annurev.bb.13.060184.000245.
9
Structure and polymorphism of the hydrocarbon chains of lipids: a study of lecithin-water phases.脂质烃链的结构与多态性：卵磷脂 - 水相的研究
J Mol Biol. 1973 Apr 25;75(4):711-33. doi: 10.1016/0022-2836(73)90303-3.
10
The thermal properties of dilauryl phosphatidylethanolamine liposomes are affected by lipid source and preparation.
Chem Phys Lipids. 1985 Nov-Dec;38(4):387-90. doi: 10.1016/0009-3084(85)90032-5.
Zotero 插件