Xiao Chuqiao, Zhou Liuyang, Gao Jie, Jia Ruibo, Zheng Yang, Zhao Suqing, Zhao Mouming, Toldrá Fidel
School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, China; Chaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory, Chaozhou, 521000, China.
School of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China.
Food Chem Toxicol. 2023 Apr;174:113652. doi: 10.1016/j.fct.2023.113652. Epub 2023 Feb 9.
Alcohol-induced liver injury has become a leading risk for human health, however, effective strategies for the prevention or treatment are still lacking. Hence, the present study explored the potential of Musculus senhousei as a source of hepatoprotective peptides against alcoholic liver injury using in vitro, in vivo and in silico methods. Results indicated that Musculus senhousei peptides (MSP, extracted by simulated gastrointestinal digestion of cooked mussel) exhibited notable antioxidant (ABTS and DPPH assays) and alcohol dehydrogenase (ADH) stabilizing activity in vitro. The ingestion of MSP markedly alleviated alcohol-induced liver injury in mice, as indicated by the decrease of serum transaminases (AST and ALT). In line with in vitro assays, significantly increased hepatic ADH activity and activated antioxidative defense system (GSH, SOD, GSH-Px and CAT) were observed, whereas the oxidative stress (MDA) was decreased. Peptidomic analysis revealed over 6000 peptides with favorable amino acid compositions, and a total of 20 potentially novel peptides with bioactivity and bioavailability were excavated among 746 of the most influential peptides using an in silico strategy. Peptides (i.e. WLPMKL, WLWLPA, RLC and RCL) were further synthesized and validated in vitro to be bioactive. These findings suggest that Musculus senhousei can be an ideal source of bioactive peptides for the prevention of alcoholic liver injury.
酒精性肝损伤已成为人类健康的主要风险因素，然而，仍缺乏有效的预防或治疗策略。因此，本研究采用体外、体内和计算机模拟方法，探索了贻贝作为抗酒精性肝损伤肝保护肽来源的潜力。结果表明，贻贝肽（通过模拟胃肠道消化熟贻贝提取的MSP）在体外表现出显著的抗氧化（ABTS和DPPH测定）和乙醇脱氢酶（ADH）稳定活性。摄入MSP可显著减轻小鼠酒精性肝损伤，血清转氨酶（AST和ALT）降低表明了这一点。与体外试验一致，观察到肝脏ADH活性显著增加，抗氧化防御系统（GSH、SOD、GSH-Px和CAT）被激活，而氧化应激（MDA）降低。肽组学分析揭示了6000多种具有良好氨基酸组成的肽，使用计算机模拟策略在746种最具影响力的肽中总共挖掘出20种具有生物活性和生物利用度的潜在新型肽。肽（即WLPMKL、WLWLPA、RLC和RCL）进一步合成并在体外验证具有生物活性。这些发现表明，贻贝可以成为预防酒精性肝损伤的理想生物活性肽来源。
