Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, China.
Guangzhou Institute of Food Inspection, Guangzhou, 510410, China.
Anal Chim Acta. 2023 Mar 15;1246:340898. doi: 10.1016/j.aca.2023.340898. Epub 2023 Jan 25.
Fenpropathrin (FPT) is a typical pyrethroid pesticide that can cause chronic toxicity to humans. Herein, an anti-FPT monoclonal antibody (mAb) was elicited via a novel hapten synthesized by introducing a carboxyl-containing spacer arm in the cyclopropane moiety of FPT. Characterized by enzyme-linked immunosorbent assay (ELISA), the mAb exhibited high affinity and selectivity to FPT with a half-maximal inhibitory concentration of 31.05 μg/L and negligible cross-reactivities with analogs of pyrethroids. Based on the mAb, a fluorescence immunochromatographic assay (FICA) for FPT detection was firstly developed. The detection limit of the FICA is 0.012 mg/kg which is much lower than the maximum residue limit of FPT for food samples. The average recoveries of FPT from spiked food samples by the FICA were 85.0-105.0%, and the obtained results were in good agreement with those of gas chromatography-tandem mass spectrometry. Overall, this work provided a reliable tool suitable for the detection of FPT residue for large-scale samples in a rapid and cost-effective manner.
烯丙菊酯（FPT）是一种典型的拟除虫菊酯类农药，对人体具有慢性毒性。在此，通过在 FPT 的环丙烷部分引入含羧基的间隔臂来合成一种新型半抗原，从而引发了针对 FPT 的单克隆抗体（mAb）。通过酶联免疫吸附测定（ELISA）进行表征，该 mAb 对 FPT 表现出高亲和力和选择性，半抑制浓度为 31.05μg/L，与拟除虫菊酯类似物的交叉反应性可忽略不计。基于该 mAb，首次开发了用于 FPT 检测的荧光免疫层析分析（FICA）。FICA 的检测限为 0.012mg/kg，远低于食品中 FPT 的最大残留限量。通过 FICA 从添加的食物样品中回收 FPT 的平均回收率为 85.0-105.0%，所得结果与气相色谱-串联质谱法的结果吻合良好。总的来说，这项工作提供了一种可靠的工具，适用于快速、经济有效地检测大规模样品中的 FPT 残留。
