Key Laboratory of Testing and Tracing of Rare Earth Products for State Market Regulation, Faculty of Materials Metallurgy and Chemistry, Jiangxi University of Science and Technology, Ganzhou, 341000, People's Republic of China.
Jiangxi Provincial Key Laboratory of Functional Molecular Materials Chemistry, Faculty of Materials Metallurgy and Chemistry, Jiangxi University of Science and Technology, Ganzhou, 341000, People's Republic of China.
Anal Bioanal Chem. 2023 Apr;415(9):1751-1764. doi: 10.1007/s00216-023-04576-z. Epub 2023 Feb 11.
Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) studies on trace element concentration and their spatial distribution in CaCO-matrix urinary stones are important but powerfully rely on matrix-matched external calibration. In this work, CaCO precipitate CaOx-1 which was doped with Mg, Cr, Mn, Fe, Co, Cu, Zn, and Sr was prepared by the homogeneous co-precipitation method. It had a homogeneous distribution of major (RSD of 0.46%) and trace elements (RSD of 1.83-6.92%) due to the negligible concentration difference compared with that prepared by the heterogeneous co-precipitation method. Based on this, an analytical method for quantitative determination of elemental concentration in CaCO-matrix samples was established using CaOx-1 as a calibration standard, and the accuracy of this method was assessed by calibrating the elemental concentration in another synthetic CaCO precipitate CaOx-2 with relative deviation (D) from - 11.43% (Mn) to 9.76% (Mg). Finally, a methodology for quantitative imaging of Mg, Cr, Mn, Fe, Co, Cu, Zn, and Sr in urinary stones via LA-ICP-MS was developed. From the elemental distributional maps, an annular texture can be found for Mg, Cu, Zn, and Sr, which corresponds to the annular white and brown texture in the real urinary stone. A homogeneous distribution of Fe and low concentrations of Cr and Co were found throughout the stone, while Mn was highly concentrated in the margin of the stone. All these results demonstrate that quantitative distribution patterns of Mg, Cr, Mn, Fe, Co, Cu, Zn, and Sr can be obtained by LA-ICP-MS using CaOx-1 as a calibration standard, which can provide potential evidence for urological and other medical studies.
激光烧蚀-电感耦合等离子体质谱（LA-ICP-MS）研究碳酸钙基质尿石中微量元素的浓度及其空间分布非常重要，但强烈依赖于基质匹配的外部校准。在这项工作中，通过均相共沉淀法制备了掺杂有 Mg、Cr、Mn、Fe、Co、Cu、Zn 和 Sr 的 CaCO3 沉淀物 CaOx-1，由于与非均相共沉淀法相比，其主要元素（RSD 为 0.46%）和微量元素（RSD 为 1.83-6.92%）的浓度差异可以忽略不计，因此具有均匀的分布。基于此，建立了一种使用 CaOx-1 作为校准标准定量测定碳酸钙基质样品中元素浓度的分析方法，并通过用相对偏差（D）从-11.43%（Mn）至 9.76%（Mg）校准另一种合成碳酸钙沉淀 CaOx-2 中的元素浓度来评估该方法的准确性。最后，通过 LA-ICP-MS 开发了一种定量成像尿石中 Mg、Cr、Mn、Fe、Co、Cu、Zn 和 Sr 的方法。从元素分布图谱中，可以发现 Mg、Cu、Zn 和 Sr 存在环形纹理，与真实尿石中的环形白色和棕色纹理相对应。整个石头中 Fe 均匀分布且 Cr 和 Co 浓度较低，而 Mn 则高度集中在石头的边缘。所有这些结果表明，通过 LA-ICP-MS 使用 CaOx-1 作为校准标准，可以获得 Mg、Cr、Mn、Fe、Co、Cu、Zn 和 Sr 的定量分布模式，这可为泌尿科和其他医学研究提供潜在证据。
