Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Faculty of Agriculture and Marshlands, University of Thi-qar, Thi-qar, Iraq.
Lipids Health Dis. 2023 Feb 10;22(1):23. doi: 10.1186/s12944-023-01788-1.
Obesity is a life-threatening metabolic disorder that predisposes individuals to other diseases. In this study, the effect of nisin, a bacteriocin produced by some bacteria, on an animal model of obesity based on selected parameters was investigated. Forty Swiss NIH mice were randomly divided into four groups and received either a placebo (saline) or nisin (25, 50, or 100 μg/kg, ip) daily for 8 weeks. The mice in all groups were fed a high-sugar diet throughout the experiment. Bodyweight and food intake were measured weekly, and at the end of the experiment, the levels of FBS, serum triglyceride, cholesterol, high-density lipoprotein, low-density lipoprotein, and hepatic enzymes were tested, and red and white blood cell counts, hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration were determined. Finally, the expression levels of some obesity-related genes, including stearoyl-CoA desaturase-1 (SCD-1), glucose transporter-4 (GLUT4), zinc finger protein 423 (zfp423), 422 (ap2), and tumor necrosis factor-alpha (TNF-α), were assessed using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). After the experiment, the body weights, abdominal fat, and body mass index were significantly lower in the nisin-treated groups than in the control group. The highest effect was observed with 50 μg/kg nisin. The expression of SCD-1, GLUT4, 422(ap2), and TNF-α decreased significantly following treatment with nisin. No significant differences were observed in the other studied parameters, and no toxic effects were observed for nisin under these experimental conditions. The results suggested that nisin could have antiobesity effects.
肥胖是一种危及生命的代谢紊乱疾病，使个体易患其他疾病。在这项研究中，研究了某些细菌产生的细菌素乳链菌肽对基于选定参数的肥胖动物模型的影响。将 40 只瑞士 NIH 小鼠随机分为四组，每天分别给予安慰剂（生理盐水）或乳链菌肽（25、50 或 100μg/kg，ip），共 8 周。所有组的小鼠在整个实验过程中都喂食高糖饮食。每周测量体重和食物摄入量，在实验结束时，测试 FBS、血清甘油三酯、胆固醇、高密度脂蛋白、低密度脂蛋白和肝酶水平，并测定红细胞和白细胞计数、血红蛋白浓度、平均红细胞体积、平均红细胞血红蛋白和平均红细胞血红蛋白浓度。最后，使用逆转录定量聚合酶链反应（RT-qPCR）评估一些与肥胖相关的基因的表达水平，包括硬脂酰辅酶 A 去饱和酶-1（SCD-1）、葡萄糖转运蛋白-4（GLUT4）、锌指蛋白 423（zfp423）、422（ap2）和肿瘤坏死因子-α（TNF-α）。实验后，与对照组相比，乳链菌肽处理组的体重、腹部脂肪和体重指数显著降低。用 50μg/kg 乳链菌肽观察到最高的效果。乳链菌肽处理后 SCD-1、GLUT4、422（ap2）和 TNF-α 的表达明显降低。在其他研究的参数中未观察到显著差异，并且在这些实验条件下未观察到乳链菌肽的毒性作用。结果表明，乳链菌肽可能具有抗肥胖作用。
