一种基于金纳米粒子的快速免疫层析法用于测定果蔬中的吡虫啉。
A Rapid Immunochromatographic Method Based on Gold Nanoparticles for the Determination of Imidacloprid on Fruits and Vegetables.
作者信息
Suryoprabowo Steven, Wu Aihong, Liu Liqiang, Kuang Hua, Xu Chuanlai, Guo Lingling
机构信息
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
出版信息
Foods. 2023 Jan 23;12(3):512. doi: 10.3390/foods12030512.
Imidacloprid (IMP) is toxic and a potential carcinogen that is most widely used as an insecticide for pest control and seed treatment. It is important to produce a rapid and sensitive assay for on-site monitoring. We have developed a novel lateral flow assay (LFA) using a sensitive monoclonal antibody (mAb) for monitoring IMP residues on fruits and vegetables. The 50% inhibition concentration result that was found when using the ELISA method was 0.247 ng mL, with the cut-off limits using the LFA method the result was 10 ng mL (0.01 M PBS), and in the samples it was 20 ng mL (with a recovery rate of 96-104.7% for Chinese cabbage, cowpea, apple, and pear samples, respectively). All of the results can be determined within seven minutes. The proposed LFA method is a valid, quick, and stable assay for the on-site detection of IMP in large numbers of samples.
吡虫啉（IMP）具有毒性且是一种潜在致癌物，它作为杀虫剂被最广泛地用于害虫防治和种子处理。开发一种用于现场监测的快速灵敏检测方法很重要。我们利用一种灵敏的单克隆抗体（mAb）开发了一种新型的侧向流动分析法（LFA），用于监测水果和蔬菜上的IMP残留。使用酶联免疫吸附测定（ELISA）方法时发现的50%抑制浓度结果为0.247 ng/mL，使用LFA方法时的截断限，在0.01 M磷酸盐缓冲盐溶液（PBS）中结果为10 ng/mL，在样品中为20 ng/mL（大白菜、豇豆、苹果和梨样品的回收率分别为96 - 104.7%）。所有结果均可在7分钟内得出。所提出的LFA方法是一种用于大量样品中IMP现场检测的有效、快速且稳定的检测方法。
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