State Key Laboratory of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Anal Methods. 2021 Sep 16;13(35):3970-3977. doi: 10.1039/d1ay01149a.
Zearalenone (ZEN) is a kind of estrogen-like mycotoxin which contaminates primary crops and their products under natural conditions and becomes a serious hazard to human health. In this study, we prepared a sensitive and specific anti-ZEN monoclonal antibody (mAb) belonging to the IgG2b subclass, with a 50%-inhibitory concentration of 0.034 ng mL. A lateral flow fluorescence microsphere immunochromatographic test strip (FM-ICTS) for the rapid and ultrasensitive detection of zearalenone in corn and wheat samples was developed based on this mAb. After optimizing experimental parameters, the visual limit of detection (LOD) of the strip assay in both corn and wheat samples was 2.5 ng mL, and the cut-off value was 25 ng mL. The LOD was calculated to be 0.68 ng mL in corn samples and 0.48 ng mL in wheat samples. Recovery experiments showed that the test results of the strip were consistent with those of ic-ELISA. As a result, this FM-ICTS assay is reliable, simple and sensitive, and can be used for rapid detection of ZEN in corn and wheat.
玉米赤霉烯酮（ZEN）是一种天然存在于农作物及其制品中的类雌激素真菌毒素，对人类健康构成严重威胁。本研究制备了一种灵敏、特异的 IgG2b 亚型抗 ZEN 单克隆抗体（mAb），其 50%抑制浓度为 0.034ng/mL。基于该 mAb，建立了用于快速灵敏检测玉米和小麦中玉米赤霉烯酮的侧向流动荧光微球免疫层析测试条（FM-ICTS）。优化实验参数后，该条带法在玉米和小麦样品中的目视检测限（LOD）分别为 2.5ng/mL 和 25ng/mL，截断值为 25ng/mL。玉米样品中的 LOD 计算值为 0.68ng/mL，小麦样品中的 LOD 计算值为 0.48ng/mL。回收实验表明，条带的检测结果与 ic-ELISA 的检测结果一致。因此，该 FM-ICTS 分析方法可靠、简单、灵敏，可用于玉米和小麦中 ZEN 的快速检测。
