Molinari Giovanni S, Wojno Michal, Terova Genciana, Wick Macdonald, Riley Hayden, Caminiti Jeffrey T, Kwasek Karolina
Center for Fisheries, Aquaculture, and Aquatic Sciences, Southern Illinois University, Carbondale, IL 62901, USA.
Department of Biotechnology and Life Sciences, University of Insubria, Via Dunant, 3-21100 Varese, Italy.
Animals (Basel). 2023 Jan 21;13(3):373. doi: 10.3390/ani13030373.
This study's objectives were to determine the effect of Largemouth Bass (LMB) muscle hydrolysates obtained using same-species digestive enzymes and the degree of LMB muscle hydrolysis when included in the first feeds of growth performance and survival, skeletal development, intestinal peptide uptake, and muscle-free amino acid composition of larval LMB. LMB muscle was mixed with digestive enzymes from adult LMB, and hydrolyzed for 1.5, 3, and 6 h, respectively. Five diets were produced, the intact diet containing non-hydrolyzed muscle and four diets with 37% muscle hydrolysate inclusion. Those diets were characterized by their level of each hydrolysate (presented as a ratio of 1.5, 3, and 6 Ts hydrolysates): 1:1:1, 1:3:6, 1:3:1, 6:3:1 for diets A, B, C, and D, respectively. To account for gut development, one group of larval LMB was fed a weekly series of diets B, C, and D to provide an increasing molecular weight profile throughout development. This group was compared against others that received either; (1) diets D, C, and B; (2) diet A; or (3) intact diet. The initial inclusion of the hydrolysates significantly improved the total length of the larval LMB; however, neither the hydrolysate inclusion nor the series of dietary molecular weight profiles improved the overall growth of larval LMB. The inclusion of hydrolysates significantly decreased the occurrence of skeletal deformities. The degree of hydrolysis did not have a significant effect on the parameters measured, except for intestinal peptide uptake, which was increased in the group that received the most hydrolyzed diet at the final time of sampling. The lack of overall growth improvement suggests that while the hydrolysates improve the initial growth performance, further research is necessary to determine the optimal molecular weight profile, hydrolysate inclusion level, and physical properties of feeds for larval LMB.
本研究的目的是确定使用同物种消化酶获得的大口黑鲈（LMB）肌肉水解产物的效果，以及将其添加到初饲料中时对大口黑鲈幼鱼生长性能、存活、骨骼发育、肠道肽吸收和肌肉游离氨基酸组成的水解程度。将大口黑鲈肌肉与成年大口黑鲈的消化酶混合，分别水解1.5、3和6小时。制备了五种饲料，完整饲料包含未水解的肌肉，另外四种饲料含有37%的肌肉水解产物。这些饲料的特征在于每种水解产物的水平（表示为1.5、3和6小时水解产物的比例）：饲料A、B、C和D的比例分别为1:1:1、1:3:6、1:3:1、6:3:1。为了考虑肠道发育，一组大口黑鲈幼鱼每周喂食一系列饲料B、C和D，以在整个发育过程中提供分子量递增的饲料。将该组与其他组进行比较，其他组分别接受：（1）饲料D、C和B；（2）饲料A；或（3）完整饲料。水解产物的初始添加显著提高了大口黑鲈幼鱼的全长；然而，水解产物的添加和饲料分子量分布系列均未改善大口黑鲈幼鱼的整体生长。水解产物的添加显著降低了骨骼畸形的发生率。水解程度对所测量的参数没有显著影响，但肠道肽吸收除外，在采样末期接受水解程度最高的饲料的组中，肠道肽吸收增加。整体生长未得到改善表明，虽然水解产物改善了初始生长性能，但仍需要进一步研究以确定大口黑鲈幼鱼饲料的最佳分子量分布、水解产物添加水平和物理性质。
