Turcu Maria-Carmen, Paștiu Anamaria Ioana, Bel Lucia Victoria, Pusta Dana Liana
Department of Genetics and Hereditary Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania.
New Companion Animals Veterinary Clinic, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania.
Animals (Basel). 2023 Feb 2;13(3):525. doi: 10.3390/ani13030525.
The early age determinism of the sex in case of monomorphic birds is very important, because most companion birds have no distinct sexual dimorphic traits. Molecular genetic sexing was proved to be one of the most accurate sex determinations in monomorphic birds. The aim of this study was to compare the results obtained by PCR performed on isolate genomic DNA from paired samples of feathers and oral swabs collected from the same individuals. Samples of oral swabs ( = 101) and feathers ( = 74) were collected from 101 companion birds from four different species (, , and spp.). The PCR was performed for the amplification of the CHD1W and CHD1Z genes in females and the CHD1Z gene in males. The overall PCR success rate of sex determination was significantly higher from oral swabs than from feathers. The PCR success rate from oral swabs was higher in juveniles and from feathers was significantly higher in adults. The similarity between the oral swab and feathers was obtained in 78.38% of the birds. Oral swabs proved to be a more reliable sample for genetic sex determination in the species tested in this study.
对于单态性鸟类而言，早期性别决定非常重要，因为大多数伴侣鸟没有明显的性二态特征。分子遗传性别鉴定被证明是单态性鸟类中最准确的性别鉴定方法之一。本研究的目的是比较通过聚合酶链反应（PCR）对从同一鸟类个体采集的成对羽毛和口腔拭子样本的分离基因组DNA进行检测所获得的结果。从101只来自四个不同物种（鹦鹉属、虎皮鹦鹉属、牡丹鹦鹉属和玄凤鹦鹉属）的伴侣鸟中采集了口腔拭子样本（n = 101）和羽毛样本（n = 74）。对雌性的CHD1W和CHD1Z基因以及雄性的CHD1Z基因进行PCR扩增以进行性别鉴定。通过口腔拭子进行性别鉴定的总体PCR成功率显著高于通过羽毛进行鉴定的成功率。在幼鸟中，通过口腔拭子进行PCR的成功率更高，而在成鸟中，通过羽毛进行PCR的成功率显著更高。78.38%的鸟类的口腔拭子和羽毛样本结果相似。在本研究中测试的物种中，口腔拭子被证明是用于遗传性别鉴定的更可靠样本。
