Biophysics and Nanosystems Laboratory, Department of Physics, Federal University of Maranhão, São Luis 65065690, MA, Brazil.
Laboratory of Nanoradiopharmacy and Synthesis of New Radiopharmaceuticals, Brazilian Nuclear Energy Commission, Nuclear Engineering Institute, Rio de Janeiro 21941906, RJ, Brazil.
Cells. 2023 Jan 31;12(3):451. doi: 10.3390/cells12030451.
The use of alpha-particle (α-particle) radionuclides, especially [Ra]RaCl (radium dichloride), for targeted alpha therapy is steadily increasing. Despite the positive clinical outcomes of this therapy, very little data are available about the effect on the ultrastructure of cells. The purpose of this study was to evaluate the nanomechanical and ultrastructure effect of [Ra] RaCl on cancer cells. To analyze the effect of [Ra]RaCl on tumor cells, human breast cancer cells (lineage MDA-MB-231) were cultured and treated with the radiopharmaceutical at doses of 2 µCi and 0.9 µCi. The effect was evaluated using atomic force microscopy (AFM) and transmission electron microscopy (TEM) combined with Raman spectroscopy. The results showed massive destruction of the cell membrane but preservation of the nucleus membrane. No evidence of DNA alteration was observed. The data demonstrated the formation of lysosomes and phagosomes. These findings help elucidate the main mechanism involved in cell death during α-particle therapy.
利用 α-粒子（α-particle）放射性核素，特别是 [Ra]RaCl（氯化镭），进行靶向 α 治疗的应用正在稳步增加。尽管这种治疗方法具有积极的临床效果，但关于其对细胞超微结构影响的数据却很少。本研究旨在评估 [Ra]RaCl 对癌细胞的纳米力学和超微结构影响。为了分析 [Ra]RaCl 对肿瘤细胞的影响，培养人乳腺癌细胞（MDA-MB-231 系）并以 2 µCi 和 0.9 µCi 的放射性药物剂量进行处理。使用原子力显微镜（AFM）和透射电子显微镜（TEM）结合拉曼光谱对其效果进行评估。结果表明，细胞膜受到严重破坏，但核膜得以保留。未观察到 DNA 改变的证据。数据表明溶酶体和吞噬体的形成。这些发现有助于阐明在 α-粒子治疗期间细胞死亡的主要机制。
