Goldman D, Lister R G, Crabbe J C
Laboratory on Clinical Studies, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892.
Brain Res. 1987 Sep 15;420(2):220-6. doi: 10.1016/0006-8993(87)91241-8.
In the mouse, there is evidence that a single genetic locus is a major determinant of differences in ethanol intake between some preferring and non-preferring inbred strains. In this report, we present evidence from two independent experiments indicating that this locus maps to chromosome 1 and that its expressed product is the abundant protein LTW-4 (a 28 kDa, pI 5.6 protein expressed in brain, liver and kidney). The genetic association was found using a panel of 14 polypeptides of mouse brain which were visualized by two-dimensional electrophoresis and which exhibit genetic variation in isoelectric point. Fifteen BXD recombinant inbred strains and the two parental strains were typed for these loci and also tested for ethanol acceptance. Strains exhibiting the basic allele showed significantly higher ethanol acceptance. When 19 distantly related inbred mouse strains were tested for ethanol acceptance and typed for LTW-4, it was again found that strains exhibiting the basic allele showed significantly higher ethanol acceptance.
在小鼠中，有证据表明，单一基因座是一些嗜酒和不嗜酒近交系之间乙醇摄入量差异的主要决定因素。在本报告中，我们提供了来自两个独立实验的证据，表明该基因座定位于1号染色体，其表达产物是丰富的蛋白质LTW-4（一种在脑、肝和肾中表达的28 kDa、pI 5.6的蛋白质）。通过二维电泳可视化的一组14种小鼠脑多肽发现了这种遗传关联，这些多肽在等电点上表现出遗传变异。对15个BXD重组近交系和两个亲本系进行了这些基因座的分型，并测试了它们对乙醇的接受程度。表现出基本等位基因的品系对乙醇的接受程度明显更高。当对19个远缘近交小鼠品系进行乙醇接受程度测试并对LTW-4进行分型时，再次发现表现出基本等位基因的品系对乙醇的接受程度明显更高。
