Goldman D, Pikus H J
Biochem Genet. 1986 Apr;24(3-4):183-94. doi: 10.1007/BF00502787.
With the description here of variant proteins A13 (pI 5.9, MW 62 kd) and A14 (pI 5.3, MW 26 kd), 14 polypeptides of mouse brain visualized by two-dimensional electrophoresis (2DE) exhibit genetic variation in isoelectric point. Using 22 B X D recombinant inbred strains, we map four of these loci and show that a fifth is independent of known loci. A pI 5.6, 81-kd protein of mouse brain mitochondria designated A1 is demonstrated to be an independent locus closely linked to LY-2 and LVP-1 on mouse chromosome 6. A pI 5.6, 28-kd genetically variant brain polypeptide designated A12 maps to chromosome 1 and shows identity with the known mouse locus LTW-4. The locus for A8 is not closely linked to any previously mapped locus. However, the locus for the newly described variant A13 shows 3 of 18 recombinants with the DNA polymorphism RN7S-2 and 2 of 18 recombinants with HC (hemolytic complement) and is thus probably located proximally to HC near the centromere of chromosome 2. Genetic and biochemical evidence is presented for the identification of A14 as ALP-1 (apolipoprotein 1), mapping to chromosome 9. In addition to these 13 genetically variant polypeptides, the positions of 12 other polypeptides which have been identified on 2DE gels of mouse brain are given.
通过这里对变异蛋白A13（pI 5.9，分子量62 kd）和A14（pI 5.3，分子量26 kd）的描述，二维电泳（2DE）显示的小鼠脑内14种多肽在等电点上呈现遗传变异。利用22个BXD重组近交系，我们对其中4个位点进行了定位，并表明第5个位点独立于已知位点。小鼠脑线粒体中一种pI 5.6、81-kd的蛋白A1被证明是一个独立位点，与小鼠6号染色体上的LY-2和LVP-1紧密连锁。一种pI 5.6、28-kd的脑遗传变异多肽A12定位于1号染色体，与已知的小鼠位点LTW-4相同。A8的位点与任何先前定位的位点都不紧密连锁。然而，新描述的变异体A13的位点与DNA多态性RN7S-2有18个重组体中的3个，与HC（溶血补体）有18个重组体中的2个，因此可能位于2号染色体着丝粒附近HC的近端。提供了遗传和生化证据以鉴定A14为ALP-1（载脂蛋白1），定位于9号染色体。除了这13种遗传变异多肽外，还给出了在小鼠脑2DE凝胶上已鉴定的其他12种多肽的位置。
