Institute of Biomedical Chemistry, 10, Pogodinskaya St., 119121 Moscow, Russia.
Int J Mol Sci. 2023 Jan 18;24(3):1877. doi: 10.3390/ijms24031877.
The beginning of the twenty-first century witnessed novel breakthrough research directions in the life sciences, such as genomics, transcriptomics, translatomics, proteomics, metabolomics, and bioinformatics. A newly developed single-molecule approach addresses the physical and chemical properties and the functional activity of single (individual) biomacromolecules and viral particles. Within the alternative approach, the combination of "single-molecule approaches" is opposed to "omics approaches". This new approach is fundamentally unique in terms of its research object (a single biomacromolecule). Most studies are currently performed using postgenomic technologies that allow the properties of several hundreds of millions or even billions of biomacromolecules to be analyzed. This paper discusses the relevance and theoretical, methodological, and practical issues related to the development potential of a single-molecule approach using methods based on molecular detectors.
二十一世纪初，生命科学领域出现了一些新的突破性研究方向，如基因组学、转录组学、转位组学、蛋白质组学、代谢组学和生物信息学。一种新开发的单分子方法可以解决单个（个体）生物大分子和病毒颗粒的物理化学性质和功能活性问题。在替代方法中，“单分子方法”的组合与“组学方法”相对立。从研究对象（单个生物大分子）的角度来看，这种新方法具有根本的独特性。目前，大多数研究都是使用后基因组技术进行的，这些技术可以分析数亿甚至数十亿个生物大分子的性质。本文讨论了使用基于分子探测器的方法发展单分子方法的相关性和理论、方法和实践问题。
