Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an 710062, China.
Int J Mol Sci. 2023 Jan 21;24(3):2146. doi: 10.3390/ijms24032146.
B-box (BBX) is a type of zinc finger transcription factor that contains a B-box domain. BBX transcription factors play important roles in plant photomorphogenesis, signal transduction, as well as abiotic and biological stress responses. However, the gene family of has not been systematically investigated to date. For this study, based on the genomic data of , 27 genes were identified and clustered into five evolutionary branches according to phylogenetic analysis. The promoter analysis suggested that may be involved in the regulation of the light responses, hormones, stress signals, and tissue-specific development. Based on the transcriptome data, the expression patterns of under different abiotic stresses and plant hormones were analyzed. The results revealed that the expressions of the genes varied under different conditions and may play essential roles in growth and development. The transient expression analysis implied that , , , , and were in the nucleus. A transcriptional activation assay showed , , , and had transactivation activities, while had none. These results provided a basis for further research on the role of SmBBXs in the development of .
B-box (BBX) 是一种含有 B-box 结构域的锌指转录因子。BBX 转录因子在植物光形态建成、信号转导以及非生物和生物胁迫响应中发挥重要作用。然而，迄今为止，尚未对 的基因家族进行系统研究。在这项研究中，基于 的基因组数据，鉴定出 27 个基因，并根据系统发育分析将其聚类为五个进化分支。启动子分析表明， 可能参与光反应、激素、应激信号和组织特异性发育的调控。基于转录组数据，分析了 在不同非生物胁迫和植物激素下的表达模式。结果表明， 基因的表达在不同条件下存在差异，可能在生长和发育中发挥重要作用。瞬时表达分析表明， 、 、 、 和 位于细胞核中。转录激活测定表明 、 、 和 具有转录激活活性，而 没有。这些结果为进一步研究 SmBBXs 在 的发育中的作用提供了基础。
