Section for Transfusion Medicine, Capital Region Blood Bank, Rigshospitalet, University of Copenhagen, 2100 Copenhagen, Denmark.
CAG Center for Endotheliomics, Copenhagen University Hospital, Rigshospitalet, 2100 Copenhagen, Denmark.
Int J Mol Sci. 2023 Jan 23;24(3):2257. doi: 10.3390/ijms24032257.
In trauma patients, shock-induced endotheliopathy (SHINE) is associated with a poor prognosis. We have previously identified four metabolic phenotypes in a small cohort of trauma patients (N = 20) and displayed the intracellular metabolic profile of the endothelial cell by integrating quantified plasma metabolomic profiles into a genome-scale metabolic model (iEC-GEM). A retrospective observational study of 99 trauma patients admitted to a Level 1 Trauma Center. Mass spectrometry was conducted on admission samples of plasma metabolites. Quantified metabolites were analyzed by computational network analysis of the iEC-GEM. Four plasma metabolic phenotypes (A-D) were identified, of which phenotype D was associated with an increased injury severity score ( < 0.001); 90% (91.6%) of the patients who died within 72 h possessed this phenotype. The inferred EC metabolic patterns were found to be different between phenotype A and D. Phenotype D was unable to maintain adequate redox homeostasis. We confirm that trauma patients presented four metabolic phenotypes at admission. Phenotype D was associated with increased mortality. Different EC metabolic patterns were identified between phenotypes A and D, and the inability to maintain adequate redox balance may be linked to the high mortality.
在创伤患者中，由休克引起的内皮病变（SHINE）与预后不良相关。我们之前在一小部分创伤患者（N=20）中确定了四种代谢表型，并通过将量化的血浆代谢组学图谱整合到基因组规模的代谢模型（iEC-GEM）中，展示了内皮细胞的细胞内代谢特征。对一家 1 级创伤中心收治的 99 名创伤患者进行了回顾性观察性研究。对入院时的血浆代谢物样本进行了质谱分析。通过 iEC-GEM 的计算网络分析对量化的代谢物进行了分析。确定了四种血浆代谢表型（A-D），其中表型 D 与损伤严重程度评分增加相关（<0.001）；在 72 小时内死亡的患者中，有 90%（91.6%）具有这种表型。发现表型 A 和 D 之间的推断 EC 代谢模式存在差异。表型 D 无法维持足够的氧化还原稳态。我们证实，创伤患者在入院时表现出四种代谢表型。表型 D 与死亡率增加相关。在表型 A 和 D 之间确定了不同的 EC 代谢模式，并且无法维持足够的氧化还原平衡可能与高死亡率有关。
