Section of Botany, Biology Department, National and Kapodistrian University of Athens, 15784 Athens, Greece.
Section of Cell Biology and Biophysics, Biology Department, National and Kapodistrian University of Athens, 15784 Athens, Greece.
Int J Mol Sci. 2023 Jan 24;24(3):2317. doi: 10.3390/ijms24032317.
Polyamine oxidases (PAOs) have been correlated with numerous physiological and developmental processes, as well as responses to biotic and abiotic stress conditions. Their transcriptional regulation is driven by signals generated by various developmental and environmental cues, including phytohormones. However, the inductive mechanism(s) of the corresponding genes remains elusive. Out of the five previously characterized Arabidopsis genes, none of their regulatory sequences have been analyzed to date. In this study, a GUS reporter-aided promoter deletion approach was used to investigate the transcriptional regulation of during normal growth and development as well as under various inductive environments. contains an upstream open reading frame (uORF) and a short inter-cistronic sequence, while the integrity of both appears to be crucial for the proper regulation of gene expression. The full-length promoter contains several -acting elements that regulate the tissue-specific expression of during normal growth and development. Furthermore, a number of TFBS that are involved in gene induction under various abiotic stress conditions display an additive effect on gene expression. Taken together, our data indicate that the transcription of is regulated by multiple environmental factors, which probably work alongside hormonal signals and shed light on the fine-tuning mechanisms of regulation.
多胺氧化酶（PAOs）与许多生理和发育过程以及对生物和非生物胁迫条件的反应有关。它们的转录调控是由各种发育和环境信号（包括植物激素）产生的信号驱动的。然而，相应基因的诱导机制尚不清楚。在之前表征的五个拟南芥基因中，迄今为止尚未分析它们的任何调节序列。在这项研究中，使用 GUS 报告基因辅助启动子缺失方法来研究在正常生长和发育以及各种诱导环境下 的转录调控。 包含一个上游开放阅读框（uORF）和一个短的内含子序列，而两者的完整性对于基因表达的适当调节似乎都是至关重要的。全长启动子包含几个 - 作用元件，可调节 在正常生长和发育过程中的组织特异性表达。此外，在各种非生物胁迫条件下参与基因诱导的许多 TFBS 在基因表达上表现出累加效应。总之，我们的数据表明 的转录受多种环境因素的调控，这些因素可能与激素信号协同作用，并阐明了 调控的微调机制。
