Dayama Bhakti R, Mahadik Varsha A, Somani Deepika, Shinde Balkrishna A, Kondhare Kirtikumar R, Karthikeyan Muthukumarasamy, Kadoo Narendra Y
Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune, Maharashtra 411008 India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh 201002 India.
Physiol Mol Biol Plants. 2025 Apr;31(4):591-608. doi: 10.1007/s12298-025-01583-5. Epub 2025 May 22.
Spot blotch disease caused by poses a significant threat to wheat production. Cultivation of disease-resistant wheat genotypes appears to be the most practical approach to mitigate the impact of this devastating disease. However, the molecular responses of wheat plants during spot blotch disease progression remain poorly understood. This study employed RNA-sequencing to unravel the spatiotemporal molecular events underlying the resistance mechanism in the spot blotch susceptible and resistant wheat genotypes. This study further provides a comprehensive overview of differentially expressed transcripts through functional analysis and transcription factor identification, elucidating the biological mechanisms governing wheat- interaction. In the resistant genotype, the expression of one of the key transcription factors, , was significantly induced upon pathogen inoculation. Computational studies, electrophoretic-mobility shift assay, and yeast one-hybrid assay confirmed the interaction of the recombinant TaWRKY41 protein with W-box elements present in the promoters of plant defense-related genes. Furthermore, co-expression network analyses identified downstream genes positively correlated with , providing insights into their probable involvement in the defense response. Overall, our investigation suggests that contributes to spot blotch resistance in wheat. This knowledge can help develop new disease-resistant wheat varieties.
The online version contains supplementary material available at 10.1007/s12298-025-01583-5.
由[病原体名称未给出]引起的叶斑病对小麦生产构成重大威胁。培育抗病小麦基因型似乎是减轻这种毁灭性病害影响的最切实可行的方法。然而，小麦植株在叶斑病发展过程中的分子反应仍知之甚少。本研究采用RNA测序来揭示叶斑病易感和抗病小麦基因型中抗性机制背后的时空分子事件。本研究通过功能分析和转录因子鉴定进一步全面概述了差异表达的转录本，阐明了控制小麦与[病原体名称未给出]相互作用的生物学机制。在抗病基因型中，一种关键转录因子[转录因子名称未给出]的表达在病原体接种后显著诱导。计算研究、电泳迁移率变动分析和酵母单杂交分析证实了重组TaWRKY41蛋白与植物防御相关基因启动子中存在的W盒元件的相互作用。此外，共表达网络分析确定了与[转录因子名称未给出]呈正相关的下游基因，为它们可能参与防御反应提供了见解。总体而言，我们的研究表明[转录因子名称未给出]有助于小麦对叶斑病的抗性。这一知识有助于培育新的抗病小麦品种。
在线版本包含可在10.1007/s12298-025-01583-5获取的补充材料。
