Departamento de Morfología y Biología Celular, School of Medicine, Julián Claveria 6, 33006 Oviedo, Spain.
Instituto Universitario de Oncología del Principado de Asturias (IUOPA), University of Oviedo, 33006 Oviedo, Spain.
Int J Mol Sci. 2023 Feb 1;24(3):2717. doi: 10.3390/ijms24032717.
Nowadays, the study of cell metabolism is a hot topic in cancer research. Many studies have used 2D conventional cell cultures for their simplicity and the facility to infer mechanisms. However, the limitations of bidimensional cell cultures to recreate architecture, mechanics, and cell communication between tumor cells and their environment, have forced the development of other more realistic in vitro methodologies. Therefore, the explosion of 3D culture techniques and the necessity to reduce animal experimentation to a minimum has attracted the attention of researchers in the field of cancer metabolism. Here, we revise the limitations of actual culture models and discuss the utility of several 3D culture techniques to resolve those limitations.
如今，细胞代谢的研究是癌症研究的一个热门话题。许多研究都使用二维常规细胞培养来简化实验并推断机制。然而，二维细胞培养在重现肿瘤细胞与其环境之间的结构、力学和细胞通讯方面存在局限性，这迫使人们开发出其他更现实的体外方法。因此，3D 培养技术的爆炸式发展和将动物实验最小化的必要性引起了癌症代谢领域研究人员的关注。在这里，我们回顾了实际培养模型的局限性，并讨论了几种 3D 培养技术的实用性，以解决这些局限性。
