Nguyen Tristan, Maniyar Anuj, Sarkar Mrinmoy, Sarkar Tapasree Roy, Neelgund Gururaj M
Department of Biology, Texas A&M University, College Station, TX 77843, USA.
Department of Chemistry, Prairie View A&M University, Prairie View, TX 77446, USA.
Nanomaterials (Basel). 2023 Jan 30;13(3):556. doi: 10.3390/nano13030556.
Cancer is a current dreadful disease and the leading cause of death. Next to cardiovascular diseases, cancer is the most severe threat to human life and health. Breast cancer is the most common invasive cancer diagnosed in women. Each year about 2.3 million women are diagnosed with breast cancer. In consideration of the severity of breast cancer, herein we designed the biocompatible nanomaterials, CNTs-HAP and GR-HAP, through grafting of hydroxyapatite (HAP) to carbon nanotubes (CNTs) and graphene (GR) nanosheets. CNTs-HAP and GR-HAP have been tested for their cytotoxicity, growth and motility inhibitory effects, and their effects on the mesenchymal markers. All these demonstrated significant dose-dependent and time-dependent in vitro cytotoxicity against SUM-159 and MCF-7 breast cancer cell lines. The cell viability assay showed that the CNTs-HAP was more effective over SUM-159 cells than MCF-7 cells. It found that the increase in the concentration of GR-HAP has inhibited the clonogenic ability of breast cancer cells. The GR-HAP exhibited a substantial inhibitory effect on the cell motility of SUM-159 cell lines. It was investigated that the expression of vimentin (mesenchymal marker) was majorly reduced in SUM-159 cells by GR-HAP.
癌症是当前一种可怕的疾病，也是主要的死亡原因。仅次于心血管疾病，癌症是对人类生命和健康最严重的威胁。乳腺癌是女性中最常见的浸润性癌症。每年约有230万女性被诊断出患有乳腺癌。鉴于乳腺癌的严重性，在此我们通过将羟基磷灰石（HAP）接枝到碳纳米管（CNT）和石墨烯（GR）纳米片上，设计了生物相容性纳米材料CNTs-HAP和GR-HAP。已对CNTs-HAP和GR-HAP的细胞毒性、生长和运动抑制作用以及它们对间充质标志物的影响进行了测试。所有这些都证明了对SUM-159和MCF-7乳腺癌细胞系具有显著的剂量依赖性和时间依赖性体外细胞毒性。细胞活力测定表明，CNTs-HAP对SUM-159细胞比MCF-7细胞更有效。研究发现，GR-HAP浓度的增加抑制了乳腺癌细胞的克隆形成能力。GR-HAP对SUM-159细胞系的细胞运动具有显著的抑制作用。研究发现，GR-HAP主要降低了SUM-159细胞中波形蛋白（间充质标志物）的表达。
