Kermani Farzad, Vojdani-Saghir Arghavan, Mollazadeh Beidokhti Sahar, Nazarnezhad Simin, Mollaei Zahra, Hamzehlou Sepideh, El-Fiqi Ahmed, Baino Francesco, Kargozar Saeid
Department of Materials Engineering, Faculty of Engineering, Ferdowsi University of Mashhad (FUM), Azadi Sq., Mashhad 917794-8564, Iran.
Tissue Engineering Research Group (TERG), Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
Transl Oncol. 2022 Jun;20:101397. doi: 10.1016/j.tranon.2022.101397. Epub 2022 Mar 30.
The utilization of bioactive glasses (BGs) in cancer therapy has recently become quite promising; herein, a series of Fe-doped mesoporous 45S5-based BGs (MBGs) were synthesized via the sol-gel method in the presence of Pluronic P123 as a soft template. The physico-chemical and biological properties of the prepared glasses were well-characterized through structural assessments, thermal analyses, and electron microscopic studies. Electrochemical analyses, including cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), were also performed to investigate the actual potential of the FeO-containing MBGs in modulating the Fenton's reaction. The XRD results confirmed the glassy state of the Fe-doped samples before immersion in simulated body fluid (SBF). The prepared Fe-doped MBGs exhibited a particle size in the range of 11-86 nm, surface charge of 27-30 mV, S of 95-306 m/g, and M of 0.08 to 0.2 emu/g. The incorporation of FeO led to a negligible decrease in the bioactivity of the glasses. The CV analysis indicated that the Fe-doped MBGs could generate HO in a cathodic potential higher than -0.2 V (vs. Ag/AgCl) in the O-saturated NaSO solution. Additionally, the data of the EIS test revealed that the FeO-doped MBGs could increase the standard rate constant of Electro-Fenton's (EF) reaction up to 38.44 times as compared with the Fe-free glasses. In conclusion, Fe-doped 45S5-derived glasses may be useful in cancer therapy strategies due to their capability of activating Fenton's reaction and subsequent production of reactive oxygen species (ROS) such as OH free radicals.
生物活性玻璃（BGs）在癌症治疗中的应用近来已颇具前景；在此，在作为软模板的普朗尼克P123存在下，通过溶胶 - 凝胶法合成了一系列铁掺杂的介孔45S5基生物活性玻璃（MBGs）。通过结构评估、热分析和电子显微镜研究对制备的玻璃的物理化学和生物学性质进行了充分表征。还进行了电化学分析，包括循环伏安法（CV）和电化学阻抗谱（EIS），以研究含FeO的MBGs在调节芬顿反应中的实际潜力。XRD结果证实了铁掺杂样品在浸入模拟体液（SBF）之前的玻璃态。制备的铁掺杂MBGs的粒径在11 - 86nm范围内，表面电荷为27 - 30mV，比表面积为95 - 306m²/g，磁化强度为0.08至0.2emu/g。FeO的掺入导致玻璃生物活性的降低可忽略不计。CV分析表明，铁掺杂的MBGs在O₂饱和的Na₂SO₄溶液中，在高于 - 0.2V（相对于Ag/AgCl）的阴极电位下可以产生·OH。此外，EIS测试数据表明，与不含铁的玻璃相比，掺FeO的MBGs可将电芬顿（EF）反应的标准速率常数提高至38.44倍。总之，铁掺杂的45S5衍生玻璃因其激活芬顿反应及随后产生活性氧（ROS）如·OH自由基的能力，可能在癌症治疗策略中有用。
