Ito N, Imaida K, de Camargo J L, Takahashi S, Asamoto M, Tsuda H
First Department of Pathology, Nagoya City University Medical School.
Jpn J Cancer Res. 1988 May;79(5):573-5. doi: 10.1111/j.1349-7006.1988.tb00023.x.
The effects of D-galactosamine on induction of preneoplastic glutathione S-transferase placental form positive liver foci were investigated in F344 rats pretreated with diethylnitrosamine (DEN) in an attempt to improve the predictive value of the medium-term bioassay system developed in our laboratory. Two weeks after the initial single ip dose (200 mg/kg) of DEN, administration of test compounds was commenced simultaneously with an ip injection of D-galactosamine at a dose of 300 mg/kg body wt. All rats were then subjected to two-thirds partial hepatectomy (PH) at week 5 and sacrificed for assessment of lesion yield at week 8. Measurement and comparison of the numbers and areas of glutathione S-transferase placental form positive (GST-P+) foci per cm2 revealed a positive response to more carcinogens, including non-hepatocarcinogens, than did the same bioassay system without injection of D-galactosamine. Thus the results suggest that inclusion of this extra proliferative stimulus may improve the medium-term detection of carcinogens and modifiers.
在经二乙基亚硝胺（DEN）预处理的F344大鼠中，研究了D - 半乳糖胺对诱发癌前谷胱甘肽S - 转移酶胎盘型阳性肝灶的影响，旨在提高我们实验室开发的中期生物测定系统的预测价值。在首次腹腔注射单剂量（200 mg/kg）DEN两周后，开始给予受试化合物，同时腹腔注射剂量为300 mg/kg体重的D - 半乳糖胺。然后所有大鼠在第5周接受三分之二部分肝切除术（PH），并在第8周处死以评估病变发生率。每平方厘米谷胱甘肽S - 转移酶胎盘型阳性（GST - P +）灶的数量和面积的测量及比较显示，与未注射D - 半乳糖胺的相同生物测定系统相比，该系统对更多致癌物（包括非肝癌致癌物）呈现阳性反应。因此，结果表明加入这种额外的增殖刺激可能会改善致癌物和调节剂的中期检测。
