Department of Chemistry, Faculty of Sciences, University of Douala, Douala P.O. Box 24157, Cameroon.
Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala P.O. Box 2701, Cameroon.
Molecules. 2023 Jan 20;28(3):1078. doi: 10.3390/molecules28031078.
A phytochemical investigation of the roots of Macfad. (Rutaceae) led to the isolation of two new compounds, namely 1-formyl-5-hydroxy--methylindolin-1-ium () and decyloxycleomiscosin D (), along with ten known compounds: 1,1-dimethylpyrrolidin-1-ium-2-carboxylate (), furan-2,3-diol (), 5-methoxyseselin (), umbelliferone (), scopoletin (), citracridone I (), citracridone II (), citracridone III (), limonin () and lupeol (). The structures were determined through the comprehensive spectroscopic analysis of 1D and 2D NMR and EI- and ESI-MS, as well as a comparison with the published data. Notably, compounds and from the genus are reported here for the first time. In addition, the MeOH extract of the roots and compounds - were screened against the human adenocarcinoma alveolar basal epithelial cell line A549 and the Caucasian prostate adenocarcinoma cell line PC3 using the MTT assay. While the extract showed significant activity, with IC values of 35.2 and 38.1 µg/mL, respectively, compounds - showed weak activity, with IC values of 99.2 to 250.2 µM and 99.5 to 192.7 µM, respectively.
从 Macfad.（芸香科）的根部分离得到两种新化合物，即 1-甲酰基-5-羟基-1-甲基吲哚啉-1-翁（）和癸氧基 cleomiscosin D（），以及十种已知化合物：1,1-二甲基吡咯烷-1-翁-2-羧酸酯（）、糠醛-2,3-二醇（）、5-甲氧基芹菜素（）、伞形酮（）、东莨菪内酯（）、刺槐素 I（）、刺槐素 II（）、刺槐素 III（）、柠檬苦素（）和羽扇豆醇（）。通过 1D 和 2D NMR 以及 EI 和 ESI-MS 的综合光谱分析以及与已发表数据的比较，确定了这些化合物的结构。值得注意的是，化合物和从属中首次报道。此外，用 MTT 法对根的甲醇提取物和化合物 - 进行了筛选，以对抗人腺癌细胞系 A549 和高加索前列腺癌细胞系 PC3。虽然提取物表现出显著的活性，IC 值分别为 35.2 和 38.1 µg/mL，但化合物 - 表现出较弱的活性，IC 值分别为 99.2 至 250.2 µM 和 99.5 至 192.7 µM。
