Ríos-Ruiz Winston F, Casique-Huamanguli Roy D, Valdez-Nuñez Renzo A, Rojas-García Jose C, Calixto-García Anderson R, Ríos-Reátegui Franz, Pompa-Vásquez Danny F, Padilla-Santa-Cruz Euler
Laboratorio de Microbiología Agrícola "Raúl Ríos Reátegui", Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, Peru.
Laboratorio de Investigación en Biotecnología, Departamento Académico de Ciencias Básicas y Afines, Facultad de Ingeniería, Universidad Nacional de Barranca, Barranca 15169, Peru.
Microorganisms. 2024 May 29;12(6):1101. doi: 10.3390/microorganisms12061101.
Due to its adsorption with aluminum and iron hydroxides, phosphorus viability is low in acidic soils; thus, the aim of this study was to isolate and identify bacteria from the rhizosphere of four legumes growing in acidic soils of the Cumbaza Sub-basin, San Martín, Peru, as well as to characterize their ability to solubilize aluminum phosphate and iron phosphate. The isolation process was conducted on TSA medium and the isolates were classified based on their origin and morphocolonial characteristics, with the bacillary shape being the most frequent, followed by cocci. To assess the solubilization of aluminum and iron phosphates, the liquid medium GELP was employed. Sixteen strains were selected, among which three stood out for their effectiveness in solubilizing AlPO (Sfcv-098-02, 22.65 mg L; Sfc-093-04, 26.50 mg L; and Sfcv-041-01-2, 55.98 mg L) and one for its ability to solubilize FePO (Sfcr-043-02, 32.61 mg L). These four strains were molecularly characterized, being identified as sp., sp., and sp. Additionally, a decrease in pH was observed in the reactions, with values ranging from 5.23 to 3.29, which enhanced the phosphate of solubilization. This suggests that the selected bacteria could be used to improve phosphorus availability in agricultural soils.
由于磷与氢氧化铝和氢氧化铁发生吸附作用,在酸性土壤中磷的有效性较低;因此,本研究的目的是从秘鲁圣马丁省昆巴扎次流域酸性土壤中生长的四种豆科植物的根际分离并鉴定细菌,以及表征它们溶解磷酸铝和磷酸铁的能力。分离过程在TSA培养基上进行,分离物根据其来源和形态菌落特征进行分类,杆菌形态最为常见,其次是球菌。为了评估磷酸铝和磷酸铁的溶解情况,使用了液体培养基GELP。挑选出16个菌株,其中有3个菌株在溶解AlPO方面表现突出(Sfcv - 098 - 02,22.65 mg/L;Sfc - 093 - 04,26.50 mg/L;Sfcv - 041 - 01 - 2,55.98 mg/L),1个菌株在溶解FePO方面表现突出(Sfcr - 043 - 02,32.61 mg/L)。对这4个菌株进行了分子表征,鉴定为 属、 属和 属。此外,在反应中观察到pH值下降,范围从5.23到3.29,这增强了磷的溶解。这表明所选细菌可用于提高农业土壤中磷的有效性。