Department of Medicine, State University of New York at Buffalo, University at Buffalo, 645 Biomedical Research Building, 3435 Main Street, Buffalo, NY 14214, USA; Department of Oral Biology, State University of New York at Buffalo, University at Buffalo, Buffalo, NY 14214, USA.
Department of Oral Biology, State University of New York at Buffalo, University at Buffalo, Buffalo, NY 14214, USA.
Cell Rep. 2023 Feb 28;42(2):112120. doi: 10.1016/j.celrep.2023.112120. Epub 2023 Feb 13.
Intestinal colonization of the oral bacterium Haemophilus parainfluenzae has been associated with Crohn's disease (CD) severity and progression. This study examines the role of periodontal disease (PD) as a modifier for colonization of H. parainfluenzae in patients with CD and explores the mechanisms behind H. parainfluenzae-mediated intestinal inflammation. Fifty subjects with and without CD were evaluated for the presence of PD, and their oral and fecal microbiomes were characterized. PD is associated with increased levels of H. parainfluenzae strains in subjects with CD. Oral inoculation of H. parainfluenzae elicits strain-dependent intestinal inflammation in murine models of inflammatory bowel disease, which is associated with increased intestinal interferon-γ (IFN-γ)+ CD4+ T cells and disruption of the host hypusination pathway. In summary, this study establishes a strain-specific pathogenic role of H. parainfluenzae in intestinal inflammation and highlights the potential effect of PD on intestinal colonization by pathogenic H. parainfluenzae strains in patients with CD.
口腔菌嗜血流感菌的肠道定植与克罗恩病（CD）的严重程度和进展有关。本研究探讨了牙周病（PD）作为 CD 患者嗜血流感菌定植的修饰因子的作用，并探讨了嗜血流感菌介导的肠道炎症的机制。评估了 50 名 CD 患者和无 CD 患者的 PD 情况，并对其口腔和粪便微生物组进行了特征分析。PD 与 CD 患者中嗜血流感菌菌株水平升高有关。在炎症性肠病的小鼠模型中，口腔接种嗜血流感菌会引发菌株依赖性的肠道炎症，这与肠道干扰素-γ（IFN-γ）+CD4+T 细胞增加和宿主氢化酶途径破坏有关。总之，本研究确立了嗜血流感菌在肠道炎症中的特定菌株致病作用，并强调了 PD 对 CD 患者肠道中致病性嗜血流感菌菌株定植的潜在影响。
