Matsuba Shuichi, Maruyama-Funatsuki Wakako, Umemoto Takayuki, Kato Hideki, Kuroki Makoto, Yokogami Narifumi, Ikegaya Tomohito, Shimizu Hiroyuki, Iriki Norio
Hokkaido Agricultural Research Center, NARO (National Agriculture and Food Research Organization), 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555, Japan.
NARO Headquarters, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8517, Japan.
Breed Sci. 2022 Dec;72(5):383-388. doi: 10.1270/jsbbs.22059. Epub 2022 Dec 7.
Rice flour is useful as a substitute for wheat flour, however, to obtain fine flour, millers need special milling facilities, which increase the cost of milling. To reduce the milling cost, we developed a floury mutant line by irradiating gamma-rays to dry seeds of the japonica cultivar 'Hoshinoyume'. The line was registered as a new cultivar, 'Hoshinoko'. Genetical analysis of the floury trait was conducted using an F population derived from a cross between 'Hoshinoko' and 'Corbetti' (a japonica rice cultivar with normal endosperm), which indicated the involvement of a single recessive gene located near the RM163 marker on the long arm of rice chromosome 5, flanking identified by Kang (2005). Sequence analysis of showed a two-bp (CA) insertion in the eighth exon of in 'Hoshinoko' compared to that of 'Hoshinoyume', which led to a frameshift mutation. The CAPS-based genotype of gene completely correlated to the phenotype of endosperm in two populations. This CAPS marker could be helpful for rice breeders to develop new cultivars harboring floury endosperm of the gene.
米粉可作为小麦粉的替代品，然而，要获得精细的面粉，磨坊主需要特殊的研磨设备，这会增加研磨成本。为降低研磨成本，我们通过对粳稻品种‘星之梦’的干燥种子进行伽马射线辐照，培育出了一个粉质突变系。该品系被登记为一个新品种‘星之粉’。利用‘星之粉’与‘Corbetti’（一个胚乳正常的粳稻品种）杂交产生的F群体，对粉质性状进行了遗传分析，结果表明，该性状受位于水稻第5染色体长臂上RM163标记附近的一个单隐性基因控制，该标记侧翼由Kang（2005年）确定。与‘星之梦’相比，‘星之粉’中该基因的第八外显子有一个两碱基对（CA）的插入，导致了移码突变。该基因基于CAPS的基因型与两个群体中胚乳的表型完全相关。这个CAPS标记有助于水稻育种者培育含有该基因粉质胚乳的新品种。
