Wang Heng, Ham Tae-Ho, Im Da-Eun, Lar San Mar, Jang Seong-Gyu, Lee Joohyun, Mo Youngjun, Jeung Ji-Ung, Kim Sun Tae, Kwon Soon-Wook
Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Korea.
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Genes (Basel). 2020 Apr 24;11(4):465. doi: 10.3390/genes11040465.
Rice varieties with suitable flour-making qualities are required to promote the rice processed-food industry and to boost rice consumption. A rice mutation, Namil(SA)-flo1, produces grains with floury endosperm. Overall, grains with low grain hardness, low starch damage, and fine particle size are more suitable for use in flour processing grains with waxy, dull endosperm with normal grain hardness and a high amylose content. In this study, fine mapping found a C to T single nucleotide polymorphism (SNP) in exon 2 of the gene encoding cytosolic pyruvate phosphate dikinase (cy). The SNP resulted in a change of serine to phenylalanine acid at amino acid position 101. The gene was named (). Co-segregation analysis with the developed cleaved amplified polymorphic sequence (CAPS) markers revealed co-segregation between the floury phenotype and the . This CAPS marker could be applied directly for marker-assisted selection. Real-time RT-PCR experiments revealed that PPDK was expressed at considerably higher levels in the mutant than in the wild type during the grain filling stage. Plastid ADP-glucose pyrophosphorylase small subunit and and soluble starch synthase ( and also exhibited enhanced expression in the mutant.
为了促进大米加工食品行业发展并增加大米消费量，需要具备合适制粉品质的水稻品种。一种水稻突变体Namil(SA)-flo1产生粉质胚乳的谷粒。总体而言，低谷粒硬度、低淀粉损伤和细颗粒尺寸的谷粒更适合用于制粉加工，而糯性、无光泽胚乳且谷粒硬度正常和直链淀粉含量高的谷粒则不然。在本研究中，精细定位发现编码胞质丙酮酸磷酸双激酶（cy）的基因外显子2中有一个从C到T的单核苷酸多态性（SNP）。该SNP导致第101位氨基酸处的丝氨酸变为苯丙氨酸。该基因被命名为（）。与开发的酶切扩增多态性序列（CAPS）标记进行共分离分析，结果显示粉质表型与（）之间存在共分离。这种CAPS标记可直接用于标记辅助选择。实时RT-PCR实验表明，在灌浆期，PPDK在（）突变体中的表达水平明显高于野生型。质体ADP-葡萄糖焦磷酸化酶小亚基（）和（）以及可溶性淀粉合酶（）和（）在（）突变体中也表现出增强表达。
