Hou Rongmiao, Yang Lizhi, Wuyun Tana, Chen Shiyao, Zhang Lu
College of Landscape and Architecture, Zhejiang A&F University, Hangzhou, China.
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China.
Front Plant Sci. 2023 Jan 26;14:1080504. doi: 10.3389/fpls.2023.1080504. eCollection 2023.
Saline-alkali stress is one of the main abiotic stress factors affecting plant growth and development. is a perennial herbal medicinal plant with high values for garden application. However, its response and tolerance to saline-alkali stress is unclear. In this study, we mixed four salts (NaCl: NaSO: NaHCO: NaCO) with a concentration ratio of 1:9:9:1, and applied low (40 and 80 mM) and high (120 and 160 mM) saline-alkali stress to analyze osmotic regulation substances, antioxidant systems and the gene expression of . Along with higher saline-alkali stress, the leaf relative water content (RWC) started to decrease only from high stress, while the malondialdehyde (MDA) content in leaves decreased continuously, and the contents of proline (Pro), soluble sugar (SS) and soluble protein (SP) increased compared with control. The activities of antioxidant enzymes and the contents of non-enzymatic antioxidants were increased positively with the accumulation of superoxide anion (O ) and hydrogen peroxide (HO). For instance, the ascorbic acid-glutathione (AsA-GSH) cycle was enhanced in seedling leaves subject to saline-alkali stress. Principal Component Analysis (PCA) indicates that MDA, Pro, SS, SP, HO, O , and GSH are important indexes to evaluate the response and tolerance of to saline-alkali stress. Through RNA-Seq, a total of 474 differentially expressed genes (DEGs) were found in plant under low saline-alkaline stress (40 mM, MSA1) . control. Among them, 364 genes were up-regulated and 110 genes were down-regulated. DEGs were extensively enriched in carbohydrate transport, transferase activity, zeatin biosynthesis, ABC transporters, and spliceosome. The transcription factor family MYB, BZIP, WRKY, and NAC were related to its saline-alkali tolerance. In addition, some DEGs encode key enzymes in the processes of osmoregulation and antioxidation, including betaine aldehyde dehydrogenase (BADH), inositol monophosphatase (IMP), chloroperoxidase (CPO), thioredoxin (Trx), and germin-like protein (GLPs) were found. Overall, these findings provide new insights into the physiological changes and molecular mechanism of to saline-alkali stress and lay a foundation for application of in saline-alkali environment.
盐碱胁迫是影响植物生长发育的主要非生物胁迫因素之一。[植物名称]是一种具有较高园林应用价值的多年生草本药用植物。然而，其对盐碱胁迫的响应和耐受性尚不清楚。在本研究中，我们将四种盐（氯化钠：硫酸钠：碳酸氢钠：碳酸钠）按1:9:9:1的浓度比混合，施加低（40和80 mM）和高（120和160 mM）盐碱胁迫，以分析渗透调节物质、抗氧化系统以及[植物名称]的基因表达。随着盐碱胁迫强度的增加，叶片相对含水量（RWC）仅在高胁迫时才开始下降，而叶片中丙二醛（MDA）含量持续降低，脯氨酸（Pro）、可溶性糖（SS）和可溶性蛋白质（SP）的含量与对照相比有所增加。抗氧化酶活性和非酶抗氧化剂含量随超氧阴离子（O）和过氧化氢（HO）的积累而呈正向增加。例如，在遭受盐碱胁迫的[植物名称]幼苗叶片中，抗坏血酸-谷胱甘肽（AsA-GSH）循环增强。主成分分析（PCA）表明，MDA、Pro、SS、SP、HO、O和GSH是评估[植物名称]对盐碱胁迫响应和耐受性的重要指标。通过RNA测序，在低盐胁迫（40 mM，MSA1）下的[植物名称]中总共发现了474个差异表达基因（DEG）。与对照相比，其中364个基因上调，110个基因下调。差异表达基因广泛富集于碳水化合物转运、转移酶活性、玉米素生物合成、ABC转运蛋白和剪接体。转录因子家族MYB、BZIP、WRKY和NAC与它的耐盐碱能力有关。此外，还发现一些差异表达基因编码渗透调节和抗氧化过程中的关键酶，包括甜菜碱醛脱氢酶（BADH）、肌醇单磷酸酶（IMP）、氯过氧化物酶（CPO）、硫氧还蛋白（Trx）和类萌发素蛋白（GLP）。总体而言，这些发现为[植物名称]对盐碱胁迫的生理变化和分子机制提供了新的见解，并为[植物名称]在盐碱环境中的应用奠定了基础。
