Xia Ling, Ge Minghuan, Shan Guang, Qian Huijun, Xia Yue
Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China.
Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.
J Oncol. 2023 Feb 2;2023:1303748. doi: 10.1155/2023/1303748. eCollection 2023.
Renal cell carcinoma (RCC) is one of the top ten tumors over the world. RCC is not sensitive to radiotherapy and chemotherapy. Therefore, it is necessary to find new targets for the treatment. CircRNAs are a special type of noncoding RNAs, which play important roles in many types of cancer. In this study, we found circ_000558 was upregulated in RCC cells, and it elevated the proliferation ability of RCC cells. The relationship between miR-1225-5p and circ_000558 or ARL4C was predicted via circBank and circular RNA interactome and confirmed by dual-luciferase reporter assay. Then, the effects of circ_000558/miR-1225-5p/ARL4C on RCC cell proliferation and apoptosis were assessed by CCK-8 assay. The results revealed that the knockdown of ARL4C significantly reduced RCC cell proliferation and overexpression of circ_000558 could significantly induce RCC cell proliferation after miR-1225-5p treatment further promoted the inhibitory ability of ARL4C knockdown. Overall, our study suggested that circ_000558/miR-1225-5p/ARL4C network was related to the RCC cell proliferation. This finding could provide new targets for the treatment and prognosis of RCC.
肾细胞癌（RCC）是全球十大肿瘤之一。RCC对放疗和化疗不敏感。因此，有必要寻找新的治疗靶点。环状RNA（circRNAs）是一类特殊的非编码RNA，在多种癌症中发挥重要作用。在本研究中，我们发现circ_000558在RCC细胞中上调，并且它提高了RCC细胞的增殖能力。通过circBank和环状RNA相互作用组预测了miR-1225-5p与circ_000558或ARL4C之间的关系，并通过双荧光素酶报告基因检测进行了验证。然后，通过CCK-8检测评估circ_000558/miR-1225-5p/ARL4C对RCC细胞增殖和凋亡的影响。结果显示，敲低ARL4C可显著降低RCC细胞增殖，circ_000558的过表达可显著诱导RCC细胞增殖，而miR-1225-5p处理后进一步增强了敲低ARL4C的抑制能力。总体而言，我们的研究表明circ_000558/miR-1225-5p/ARL4C网络与RCC细胞增殖有关。这一发现可为RCC的治疗和预后提供新的靶点。
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