Bracey A W, McFarland F
Transfusion. 1984 Jul-Aug;24(4):330-2. doi: 10.1046/j.1537-2995.1984.24484275575.x.
Red cells can be fractionated by age on a blood processor (Model 2991, IBM). However, little data exist on processing a product for transfusion. This report details a method for collecting young erythrocyte (YE) units containing a mean hemoglobin content of 56.43 +/- 1.39 (SEM) grams. Enrichment with YEs was indicated by greater activity of the enzyme pyruvate kinase (PK) in aliquots of YE units. The mean ratio of PK activity in the young red cell product compared to unfractionated blood was 1.14 +/- 0.03 (SEM). An increased PK ratio was noted in 23 of 26 units studied. Based on the PK ratio, the mean age of the erythrocytes was 46 days, compared to 60 days for standard units of blood. In the 6 units assayed, the reticulocyte count was increased. Collection of YE units was relatively easy, no untoward donor reactions were observed, and no deleterious effects developed in the recipient. Although these data suggest enrichment with a young population of red cells, YE units produced by this method are experimental. Routine use of this procedure awaits demonstration of a significant decrease in transfusion frequency in patients receiving YE units for chronic red cell therapy.
红细胞可通过血液处理器（IBM 2991型）按年龄进行分离。然而，关于制备用于输血的产品的数据却很少。本报告详细介绍了一种收集平均血红蛋白含量为56.43±1.39（标准误）克的年轻红细胞（YE）单位的方法。YE单位等分试样中丙酮酸激酶（PK）的活性更高，表明富含年轻红细胞。年轻红细胞产品中PK活性与未分离血液相比的平均比值为1.14±0.03（标准误）。在所研究的26个单位中有23个单位的PK比值增加。根据PK比值，红细胞的平均年龄为46天，而标准血液单位为60天。在所检测的6个单位中，网织红细胞计数增加。收集YE单位相对容易，未观察到不良的供体反应，且受体未出现有害影响。尽管这些数据表明富含年轻红细胞群体，但通过这种方法制备的YE单位仍处于实验阶段。在接受YE单位进行慢性红细胞治疗的患者中，常规使用该程序有待证明输血频率显著降低。
