Fontes Ana Luiza, Pimentel Lígia Leão, Soares Ana Maria Silva, Domingues Maria do Rosário, Rodríguez-Alcalá Luis Miguel, Gomes Ana Maria
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal; Laboratório Associado para a Química Verde - LAQV-REQUIMTE, Departamento de Química, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Food Chem. 2023 Jul 1;413:135665. doi: 10.1016/j.foodchem.2023.135665. Epub 2023 Feb 9.
This work studied the viability of using vegetable oils as precursor substrates to develop a dairy product enriched in microbial conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids. Hydrolysis of hempseed, flaxseed (FSO) and soybean (SBO) oils was tested with Candida rugosa (CRL), Pseudomonas fluorescens, or Pancreatic porcine lipases. FSO and SBO, previously hydrolyzed with CRL, were further selected for cow's milk CLA/CLNA-enrichment with Bifidobacterium breve DSM 20091. Thereafter, higher substrate concentrations with hydrolyzed FSO were tested. For all tested oils, CRL revealed the best degrees of hydrolysis (>90 %). Highest microbial CLA/CLNA yield in milk was achieved with hydrolyzed FSO, which led to the appearance of mainly CLNA isomers (0.34 mg/g). At higher substrate concentrations, maximum yield was 0.88 mg/g CLNA. Therefore, it was possible to enrich milk with microbial CLNA using vegetable oil, but not with CLA, nor develop a functional product that can deliver a reliable effective dose.
本研究探讨了使用植物油作为前体底物来开发富含微生物共轭亚油酸（CLA）和共轭亚麻酸（CLNA）的乳制品的可行性。用皱落假丝酵母（CRL）、荧光假单胞菌或猪胰脂肪酶对大麻籽油、亚麻籽油（FSO）和大豆油（SBO）进行水解试验。先前用CRL水解的FSO和SBO，进一步用短双歧杆菌DSM 20091进行牛乳CLA/CLNA富集试验。此后，对水解FSO的更高底物浓度进行了测试。对于所有测试的油，CRL显示出最佳水解程度（>90%）。水解FSO在牛奶中获得了最高的微生物CLA/CLNA产量，这导致主要CLNA异构体的出现（0.34mg/g）。在更高的底物浓度下，最大产量为0.88mg/g CLNA。因此，有可能使用植物油在牛奶中富集微生物CLNA，但不能富集CLA，也无法开发出能提供可靠有效剂量的功能性产品。
