Wei Gang, Wu Ge, Sun Jiajia, Qi Yi, Zhao Qi, Xu Fengde, Zhang Zhi, Peng Lanzhi
School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang 524023, China.
The Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang 524023, China.
Foods. 2024 Aug 6;13(16):2472. doi: 10.3390/foods13162472.
Conjugated linoleic acid (CLA) is a class of bioactive fatty acids that exhibit various physiological activities such as anti-cancer, anti-atherosclerosis, and lipid-lowering. It is an essential fatty acid that cannot be synthesized by the human body and must be derived from dietary sources. The natural sources of CLA are limited, predominantly relying on chemical and enzymatic syntheses methods. Microbial biosynthesis represents an environmentally benign approach for CLA production. Pine nut oil, containing 40-60% linoleic acid, serves as a promising substrate for CLA enrichment. In the present study, we developed a novel method for the production of CLA from pine nut oil using () , which harbors a linoleic acid isomerase. The optimal fermentation parameters for CLA production were determined using a combination of single-factor and response surface methodologies: an inoculum size of 2%, a fermentation temperature of 36 °C, a fermentation time of 20 h, and a pine nut oil concentration of 11%. Under these optimized conditions, the resultant CLA yield was 33.47 μg/mL. Gas chromatography analysis revealed that the fermentation process yielded a mixture of c9, t11CLA and t10, c12 CLA isomers, representing 4.91% and 4.86% of the total fatty acid content, respectively.
共轭亚油酸（CLA）是一类具有多种生理活性的生物活性脂肪酸，如抗癌、抗动脉粥样硬化和降脂等。它是人体无法合成的必需脂肪酸，必须从饮食来源中获取。CLA的天然来源有限，主要依赖化学和酶促合成方法。微生物生物合成是一种环境友好的CLA生产方法。含有40%-60%亚油酸的松子油是CLA富集的一种有前景的底物。在本研究中，我们开发了一种利用（）从松子油生产CLA的新方法，（）含有亚油酸异构酶。使用单因素和响应面方法相结合的方式确定了CLA生产的最佳发酵参数：接种量2%、发酵温度36℃、发酵时间20小时和松子油浓度11%。在这些优化条件下，CLA的产量为33.47μg/mL。气相色谱分析表明，发酵过程产生了c9，t11CLA和t10，c12CLA异构体的混合物，分别占总脂肪酸含量的4.91%和4.86%。
