Lübeck Institute of Experimental Dermatology, University of Lübeck, Lübeck, Germany.
Division of Nephrology, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA.
Nat Commun. 2023 Feb 14;14(1):834. doi: 10.1038/s41467-023-36479-z.
The mammalian gut is home to a diverse microbial ecosystem, whose composition affects various physiological traits of the host. Next-generation sequencing-based metagenomic approaches demonstrated how the interplay of host genetics, bacteria, and environmental factors shape complex traits and clinical outcomes. However, the role of fungi in these complex interactions remains understudied. Here, using 228 males and 363 females from an advanced-intercross mouse line, we provide evidence that fungi are regulated by host genetics. In addition, we map quantitative trait loci associated with various fungal species to single genes in mice using whole genome sequencing and genotyping. Moreover, we show that diet and its' interaction with host genetics alter the composition of fungi in outbred mice, and identify fungal indicator species associated with different dietary regimes. Collectively, in this work, we uncover an association of the intestinal fungal community with host genetics and a regulatory role of diet in this ecological niche.
哺乳动物的肠道是一个多样化的微生物生态系统的家园，其组成影响宿主的各种生理特征。基于下一代测序的宏基因组学方法表明，宿主遗传学、细菌和环境因素的相互作用如何塑造复杂的特征和临床结果。然而，真菌在这些复杂相互作用中的作用仍未得到充分研究。在这里，我们使用来自一个先进的杂交鼠系的 228 只雄性和 363 只雌性，提供了真菌受宿主遗传学调控的证据。此外，我们使用全基因组测序和基因分型，将与各种真菌物种相关的数量性状基因座映射到小鼠中的单个基因上。此外，我们还表明，饮食及其与宿主遗传学的相互作用会改变杂交鼠中真菌的组成，并确定与不同饮食方案相关的真菌指示物种。总的来说，在这项工作中，我们揭示了肠道真菌群落与宿主遗传学的关联，以及饮食在这个生态位中的调节作用。
