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利用散斑图像亚像素相关分析揭示微生物菌落生长的机制。

Use of the speckle imaging sub-pixel correlation analysis in revealing a mechanism of microbial colony growth.

机构信息

Faculty of Computer Science and Information Technology, Department of Computer Control and Computer Networks, Riga Technical University, Zunda Krastmala 10, LV-1048, Riga, Latvia.

Laboratorija Auctoritas Ltd, Čiekurkalna 1. linija 11, Riga, LV-1026, Latvia.

出版信息

Sci Rep. 2023 Feb 14;13(1):2613. doi: 10.1038/s41598-023-29809-0.

DOI:10.1038/s41598-023-29809-0
PMID:36788263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9929235/
Abstract

The microbial colony growth is driven by the activity of the cells located on the edges of the colony. However, this process is not visible unless specific staining or cross-sectioning of the colony is done. Speckle imaging technology is a non-invasive method that allows visualization of the zones of increased microbial activity within the colony. In this study, the laser speckle imaging technique was used to record the growth of the microbial colonies. This method was tested on three different microorganisms: Vibrio natriegens, Escherichia coli, and Staphylococcus aureus. The results showed that the speckle analysis system is not only able to record the growth of the microbial colony but also to visualize the microbial growth activity in different parts of the colony. The developed speckle imaging technique visualizes the zone of "the highest microbial activity" migrating from the center to the periphery of the colony. The results confirm the accuracy of the previous models of colony growth and provide algorithms for analysis of microbial activity within the colony.

摘要

微生物菌落的生长是由位于菌落边缘的细胞活动驱动的。然而，除非对菌落进行特定的染色或切片，否则这个过程是不可见的。散斑成像技术是一种非侵入性的方法，可以观察到菌落中微生物活性增加的区域。在这项研究中，使用激光散斑成像技术来记录微生物菌落的生长。该方法在三种不同的微生物上进行了测试：嗜盐菌、大肠杆菌和金黄色葡萄球菌。结果表明，散斑分析系统不仅能够记录微生物菌落的生长，还能够可视化菌落不同部位的微生物生长活性。所开发的散斑成像技术可以可视化“最高微生物活性”区域从菌落中心向边缘迁移。结果证实了先前的菌落生长模型的准确性，并为分析菌落内的微生物活性提供了算法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/bb4704c0dfce/41598_2023_29809_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/fc23aa09b0e8/41598_2023_29809_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/d60321d6fbf9/41598_2023_29809_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/60baa8de5d61/41598_2023_29809_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/0038213afc4f/41598_2023_29809_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/4a50d7dfc7ce/41598_2023_29809_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/31b39001c7a3/41598_2023_29809_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/3d909f5a4397/41598_2023_29809_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/84c576c4751a/41598_2023_29809_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/239bc69479c8/41598_2023_29809_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/7bb29f5648a3/41598_2023_29809_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/e6986f497ab8/41598_2023_29809_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/bb4704c0dfce/41598_2023_29809_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/fc23aa09b0e8/41598_2023_29809_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/d60321d6fbf9/41598_2023_29809_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/60baa8de5d61/41598_2023_29809_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/0038213afc4f/41598_2023_29809_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/4a50d7dfc7ce/41598_2023_29809_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/31b39001c7a3/41598_2023_29809_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/3d909f5a4397/41598_2023_29809_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/84c576c4751a/41598_2023_29809_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/239bc69479c8/41598_2023_29809_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/7bb29f5648a3/41598_2023_29809_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/e6986f497ab8/41598_2023_29809_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/deca/9929235/bb4704c0dfce/41598_2023_29809_Fig12_HTML.jpg

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