在鲟鱼精液冷冻保存中使用冷冻保护剂和抗氧化剂。

Use of cryoprotectors and antioxidants in sturgeon semen cryopreservation.

机构信息

Southern Scientific Centre of Russian Academy of Science, 41 Chekhova str., 344006, Rostov-on-Don, Russia.

Astrakhan State Technical University, 16 Tatisheva str., 414056, Astrakhan, Russia.

出版信息

Cryobiology. 2023 Jun;111:30-39. doi: 10.1016/j.cryobiol.2023.02.003. Epub 2023 Feb 14.

DOI:10.1016/j.cryobiol.2023.02.003

PMID:36791902

Abstract

In recent years, the possibility of increasing the low cryoresistance of sturgeon sperm by using antioxidants in basic cryoprotective media has been studied. The goal of this work was to review the current literature on impact of the cryoprotectors, well-known antioxidants and new multifunctional compounds on the activity indicators and fertilizing capability of sperm, as well as on biomarkers of cryostress. A special attention is given to the radical scavenging activity of studied compounds in relation to the highly reactive oxygen species, to prevent and negate oxidative stress damage of sturgeon sperm. Also, new trends for future research through the application of novel polyfunctional antioxidants to sturgeon sperm cryopreservation were indicated.

摘要

近年来，人们研究了在基本的冷冻保护介质中使用抗氧化剂来提高鲟鱼精子的低抗冻性的可能性。本工作的目的是综述关于冷冻保护剂、知名抗氧化剂和新型多功能化合物对精子活力指标和受精能力以及冷冻应激生物标志物的影响的现有文献。特别关注研究化合物清除自由基的活性与高反应性氧物质的关系，以防止和消除鲟鱼精子的氧化应激损伤。此外，还指出了通过应用新型多功能抗氧化剂来进行鲟鱼精子冷冻保存的未来研究的新趋势。