Laboratory of Microorganisms and Active Biomolecules, MBA-LR03ES03, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia.
Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, Ferhat Abbas University, 19000, Setif, Algeria.
Genomics. 2023 Mar;115(2):110579. doi: 10.1016/j.ygeno.2023.110579. Epub 2023 Feb 13.
Heavy metal-tolerant plant growth-promoting bacteria (PGPB) have gained popularity in bioremediation in recent years. A genome-assisted study of a heavy metal-tolerant PGPB Pantoea eucrina OB49 isolated from the rhizosphere of wheat grown on a heavy metal-contaminated site is presented. Comparative pan-genome analysis indicated that OB49 acquired heavy metal resistance genes through horizontal gene transfer. On contigs S10 and S12, OB49 has two arsRBCH operons that give arsenic resistance. On the S12 contig, an arsRBCH operon was discovered in conjunction with the merRTPCADE operon, which provides mercury resistance. P. eucrina OB49 may be involved in an ecological alternative for heavy metal remediation and growth promotion of wheat grown in metal-polluted soils. Our results suggested the detection of mobile genetic elements that harbour the ars operon and the fluoride resistance genes adjacent to the mer operon.
近年来，耐重金属植物促生菌（PGPB）在生物修复中受到关注。本文对一株耐重金属 PGPB——小麦根际 Pantoea eucrina OB49 的全基因组进行了研究。该菌分离自重金属污染区的小麦根际。比较泛基因组分析表明，OB49 通过水平基因转移获得了重金属抗性基因。在 S10 和 S12 两个连续基因片段上，OB49 有两个 arsRBCH 操纵子，赋予砷抗性。在 S12 连续基因片段上，与 merRTPCADE 操纵子一起发现了一个 arsRBCH 操纵子，该操纵子赋予汞抗性。P. eucrina OB49 可能参与了重金属污染土壤中小麦生长的重金属修复和促生的生态替代。我们的研究结果表明，检测到了携带 ars 操纵子和 mer 操纵子附近氟化物抗性基因的移动遗传元件。
