Bak Seon Gyeong, Lim Hyung Jin, Park Eun Jae, Won Yeong Seon, Lee Seung Woong, Lee Soyoung, Park Sang-Ik, Lee Seung Jae, Rho Mun-Chual
Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 56212, South Korea.
College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, South Korea.
Heliyon. 2023 Jan 20;9(2):e12994. doi: 10.1016/j.heliyon.2023.e12994. eCollection 2023 Feb.
is an edible crop and herbal medicine that is known to have antipyretic, anti-inflammatory, and anti-edema effects. Many studies have been conducted on the 95% ethanol extract of , but there is little research on the 70% ethanol extract and hemiphloin, which is a new indicator component of the 70% ethanol extract of . To investigate the anti-atopic effect and verify the mechanism action of 70% ethanol extract of (VAE), TNF-α/IFN-γ-induced HaCaT keratinocytes were used. The VAE treatment alleviated TNF-α/IFN-γ-induced IL-1β, IL-6, IL-8, CCL17/TARC, and CCL22/MDC gene expressions and productions. VAE also inhibited the phosphorylation of MAPKs, including p38, ERK, JNK, STAT1, and NF-κB in TNF-α/IFN-γ-induced HaCaT cells. 2,4-dinitochlorobenzene (DNCB)-induced skin inflammation mice model, and HaCaT keratinocytes were used. In the DNCB-induced mouse model, VAE treatment alleviated ear thicknesses and IgE levels. Furthermore, VAE decreased IL-1β, IL-6, IL-8, CCL17/TARC, and CCL22/MDC gene expressions of DNCB-applied ear tissue. Additionally, we investigated the anti-atopic and anti-inflammatory effects of hemiphloin using TNF-α/IFN-γ-induced HaCaT keratinocytes and LPS-induced J774 macrophages. Treatment hemiphloin decreased gene expressions and productions of IL-1β, IL-6, IL-8, CCL17/TARC, and CCL22/MDC in TNF-α/IFN-γ-induced HaCaT cells. The phosphorylations of p38, ERK, STAT1, and NF-κB were inhibited by hemiphloin in TNF-α/IFN-γ-induced HaCaT cells. Finally, hemiphloin showed anti-inflammatory activities in LPS-induced J774 cells. It decreased LPS-induced NO productions and iNOS and COX-2 expressions. Treatment of hemiphloin also inhibited LPS-induced TNF-α, IL-1β, and IL-6 gene expressions. These results suggest that VAE is an anti-inflammatory agent for inflammatory skin diseases and that hemiphloin could be a therapeutic candidate for inflammatory skin diseases.
是一种可食用作物和草药，已知具有解热、抗炎和抗水肿作用。对其95%乙醇提取物已进行了许多研究，但对70%乙醇提取物以及作为70%乙醇提取物新指标成分的半韧皮部的研究较少。为了研究其抗特应性作用并验证70%乙醇提取物（VAE）的作用机制，使用了TNF-α/IFN-γ诱导的HaCaT角质形成细胞。VAE处理减轻了TNF-α/IFN-γ诱导的IL-1β、IL-6、IL-8、CCL17/TARC和CCL22/MDC基因表达及生成。VAE还抑制了TNF-α/IFN-γ诱导的HaCaT细胞中包括p38、ERK、JNK、STAT1和NF-κB在内的MAPKs的磷酸化。使用了2,4-二硝基氯苯（DNCB）诱导的皮肤炎症小鼠模型和HaCaT角质形成细胞。在DNCB诱导的小鼠模型中，VAE处理减轻了耳厚度和IgE水平。此外，VAE降低了DNCB处理的耳部组织中IL-1β、IL-6、IL-8、CCL17/TARC和CCL22/MDC的基因表达。此外，我们使用TNF-α/IFN-γ诱导的HaCaT角质形成细胞和LPS诱导的J774巨噬细胞研究了半韧皮部的抗特应性和抗炎作用。半韧皮部处理降低了TNF-α/IFN-γ诱导的HaCaT细胞中IL-1β、IL-6、IL-8、CCL17/TARC和CCL22/MDC的基因表达及生成。在TNF-α/IFN-γ诱导的HaCaT细胞中，半韧皮部抑制了p38、ERK、STAT1和NF-κB的磷酸化。最后，半韧皮部在LPS诱导的J774细胞中显示出抗炎活性。它降低了LPS诱导的NO生成以及iNOS和COX-2表达。半韧皮部处理还抑制了LPS诱导的TNF-α、IL-1β和IL-6基因表达。这些结果表明VAE是一种用于炎症性皮肤病的抗炎剂，并且半韧皮部可能是炎症性皮肤病的一种治疗候选物。
