Department of Biological Sciences, Microbiology Unit, Faculty of Science, Olusegun Agagu University of Science and Technology, PMB 353, 350105, Okitipupa, Nigeria.
Food Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa.
Lett Appl Microbiol. 2023 Feb 16;76(2). doi: 10.1093/lambio/ovac030.
The word endosphere represents the internal tissues of plants harboring diverse microbes capable of producing active biological products for various biotechnological and agricultural applications. The discreet standalone genes and interdependent association of microbial endophytes with plants can be an underlining factor in predicting their ecological functions. Yet-to-be-cultured endophytic microbes have geared the invention of metagenomics in various environmental studies to determine their structural diversity and functional genes with novel attributes. This review presents an overview of the general concept of metagenomics in microbial endophytic studies. First, the endosphere microbial communities were introduced, followed by metagenomic insights in endosphere biology, a promising technology. Also, the major application of metagenomics and a short brief on DNA stable isotope probing in determining functions and metabolic pathways of microbial metagenome were highlighted. Therefore, the use of metagenomics promises to provide answers to yet-to-be-cultured microbes by unraveling their diversity, functional attributes, and metabolic pathways with prospects in integrated and sustainable agriculture.
内共生体一词代表了植物内部组织中蕴藏着各种能够产生具有各种生物技术和农业应用价值的活性生物产物的微生物。微生物内共生体离散的独立基因和与植物的相互依存关系可能是预测其生态功能的一个潜在因素。尚未培养的内生微生物已经推动了宏基因组学在各种环境研究中的发明，以确定其具有新颖特性的结构多样性和功能基因。本综述介绍了微生物内生研究中宏基因组学的一般概念。首先介绍了内共生体微生物群落，然后介绍了内共生体生物学的宏基因组学见解，这是一项很有前途的技术。还强调了宏基因组学的主要应用以及 DNA 稳定同位素探测在确定微生物宏基因组功能和代谢途径方面的简要应用。因此，通过揭示尚未培养的微生物的多样性、功能特性和代谢途径，宏基因组学有望为综合和可持续农业提供答案。
