Song Wenchen, Wang Yao, Peng Bo, Yang Linyan, Gao Jian, Xiao Chunwang
College of Life and Environmental Sciences, Minzu University of China, Beijing, China.
Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, China.
Front Plant Sci. 2023 Dec 7;14:1297399. doi: 10.3389/fpls.2023.1297399. eCollection 2023.
Owing to challenges in the study of complex rhizosphere and endophytic microbial communities, the composition and function of such microbial communities in steppe ecosystems remain elusive. Here, we studied the microbial communities of the rhizosphere and endophytic microbes of the dominant plant species across the Inner Mongolian steppes using metagenomic sequencing and investigated their relationships with changes in mean annual temperature (MAT) and mean annual precipitation (MAP).
Metagenomic sequencing based on Illumina high-throughput sequencing, using the paired end method to construct a small fragment library for sequencing.
Adaptation of root systems to the environment affected the composition and function of rhizosphere and endophytic microbial communities. However, these communities exhibited distinct community assembly and environmental adaptation patterns. Both rhizosphere and endophytic microbial communities can be divided into two unrelated systems based on their ecological niches. The composition and function of the rhizosphere microbial communities were mainly influenced by MAT, while those of the endophytic microbial communities were mainly influenced by MAP. MAT affected the growth, reproduction, and lipid decomposition of rhizosphere microorganisms, whereas MAP affected reverse transcription and cell wall/membrane/envelope biogenic functions of endophytic microorganisms.
Our findings reveal the composition and function of the rhizosphere and endophytic microbial communities in response to changes in MAP and MAT, which has important implications for future biogeography and climate change research.
由于在研究复杂的根际和内生微生物群落方面存在挑战，草原生态系统中此类微生物群落的组成和功能仍不清楚。在这里，我们使用宏基因组测序研究了内蒙古草原上优势植物物种的根际微生物群落和内生微生物，并调查了它们与年均温度（MAT）和年平均降水量（MAP）变化的关系。
基于Illumina高通量测序的宏基因组测序，采用双端方法构建小片段文库进行测序。
根系对环境的适应性影响了根际和内生微生物群落的组成和功能。然而，这些群落表现出不同的群落组装和环境适应模式。基于生态位，根际和内生微生物群落均可分为两个不相关的系统。根际微生物群落的组成和功能主要受MAT影响，而内生微生物群落的组成和功能主要受MAP影响。MAT影响根际微生物的生长、繁殖和脂质分解，而MAP影响内生微生物的逆转录和细胞壁/膜/包膜生物合成功能。
我们的研究结果揭示了根际和内生微生物群落响应MAP和MAT变化的组成和功能，这对未来的生物地理学和气候变化研究具有重要意义。
