Horio T, Ofuji S
Acta Derm Venereol. 1976;56(5):367-71.
The distribution and fate of 3, 3', 4', 5-tetrachlorosalicylanilide (TCSA) in photosensitized guinea-pig skin following topical application at varying times was investigated by fluorescence microscopy in unfixed, frozen sections. When the site to which TCSA had been applied was irradiated with long-wave UV light, TCSA was detected in the entire epidermis only 3 days after application and in the horny layer up to 10 days. Unless TCSA-treated sites were exposed to light, TCSA was still recognized in the entire epidermis 10 days after application and in the horny layer even at 3 weeks. The irradiated photosensitized guinea pigs in the present study eliminated TCSA more rapidly than normal control animals in our previous work. The mechanism of persistent light reaction was discussed and we concluded that TCSA can remain in the skin for a long time, because it is a prohapten, not a hapten.
通过对未固定的冰冻切片进行荧光显微镜检查，研究了在不同时间局部应用后，3,3',4',5-四氯水杨酰苯胺（TCSA）在光敏化豚鼠皮肤中的分布和归宿。当用长波紫外线照射涂抹了TCSA的部位时，仅在涂抹后3天在整个表皮中检测到TCSA，在角质层中可检测到长达10天。除非将经TCSA处理的部位暴露于光下，否则在涂抹后10天仍可在整个表皮中识别出TCSA，甚至在3周时在角质层中仍可检测到。本研究中经照射的光敏化豚鼠比我们之前研究中的正常对照动物更快地消除了TCSA。讨论了持续光反应的机制，我们得出结论，TCSA可以在皮肤中长时间留存，因为它是一种前半抗原，而非半抗原。
