Department of Biomedical Engineering, Faculty of Engineering, Universiti Malaya, Kuala Lumpur, Malaysia.
Department of Biomedical Engineering, Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, Universiti Malaya, Kuala Lumpur, Malaysia.
PLoS One. 2023 Feb 16;18(2):e0280381. doi: 10.1371/journal.pone.0280381. eCollection 2023.
Diagnosing oral diseases at an early stage may lead to better preventive treatments, thus reducing treatment burden and costs. This paper introduces a systematic design of a microfluidic compact disc (CD) consisting of six unique chambers that run simultaneously from sample loading, holding, mixing and analysis. In this study, the electrochemical property changes between real saliva and artificial saliva mixed with three different types of mouthwashes (i.e. chlorhexidine-, fluoride- and essential oil (Listerine)-based mouthwashes) were investigated using electrical impedance analysis. Given the diversity and complexity of patient's salivary samples, we investigated the electrochemical impedance property of healthy real saliva mixed with different types of mouthwashes to understand the different electrochemical property which could be a foundation for diagnosis and monitoring of oral diseases. On the other hand, electrochemical impedance property of artificial saliva, a commonly used moisturizing agent and lubricant for the treatment of xerostomia or dry mouth syndrome was also studied. The findings indicate that artificial saliva and fluoride-based mouthwash showed higher conductance values compared to real saliva and two other different types of mouthwashes. The ability of our new microfluidic CD platform to perform multiplex processes and detection of electrochemical property of different types of saliva and mouthwashes is a fundamental concept for future research on salivary theranostics using point-of-care microfluidic CD platform.
早期诊断口腔疾病可能会导致更好的预防治疗，从而减轻治疗负担和成本。本文介绍了一种由六个独特腔室组成的微流控光盘（CD）的系统设计，这些腔室可以同时从样本加载、保持、混合和分析开始运行。在这项研究中，使用电阻抗分析研究了真实唾液与三种不同类型漱口水（即含氯己定、氟化物和桉叶油（李施德林）漱口水）混合后的电化学性质变化。鉴于患者唾液样本的多样性和复杂性，我们研究了不同类型漱口水混合的健康真实唾液的电化学阻抗特性，以了解可能用于口腔疾病诊断和监测的不同电化学特性。另一方面，还研究了人工唾液（一种常用于治疗口干或干燥性口炎的保湿剂和润滑剂）的电化学阻抗特性。研究结果表明，与真实唾液和另外两种不同类型的漱口水相比，人工唾液和含氟漱口水的电导率值更高。我们的新型微流控 CD 平台进行多路处理和不同类型唾液和漱口水电化学性质检测的能力，是使用即时护理微流控 CD 平台进行唾液治疗学研究的基本概念。
