School of Automation Engineering, University of Electronic Science and Technology of China, Chengdu 611731, China.
School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu 611731, China.
Analyst. 2021 Sep 27;146(19):5800-5821. doi: 10.1039/d1an00629k.
Rapid diagnosis plays a vital role in daily life and is effective in reducing treatment costs and increasing curability, especially in remote areas with limited availability of resources. Among the various common methods of rapid diagnosis, centrifugal microfluidics has many unique advantages, such as less sample consumption, more precise valve control for sequential loading of samples, and accurately separated module design in a microfluidic network to minimize cross-contamination. Therefore, in recent years, centrifugal microfluidics has been extensively researched, and it has been found to play important roles in biology, chemistry, and medicine. Here, we review the latest developments in centrifugal microfluidic platforms in immunoassays, biochemical analyses, and molecular diagnosis, in recent years. In immunoassays, we focus on the application of enzyme-linked immunosorbent assay (ELISA); in biochemical analysis, we introduce the application of plasma and blood cell separation; and in molecular diagnosis, we highlight the application of nucleic acid amplification tests. Additionally, we discuss the characteristics of the methods under each platform as well as the enhancement of the corresponding performance parameters, such as the limit of detection, separation efficiency, . Finally, we discuss the limitations associated with the existing applications and potential breakthroughs that can be achieved in this field in the future.
快速诊断在日常生活中起着至关重要的作用，它可以有效降低治疗成本并提高治愈率，特别是在资源有限的偏远地区。在各种常见的快速诊断方法中，离心微流控技术具有许多独特的优势，例如样品消耗较少、对样品顺序加载的精确阀门控制以及微流控网络中精确分离的模块设计，以最小化交叉污染。因此，近年来离心微流控技术得到了广泛的研究，并在生物学、化学和医学领域发挥了重要作用。在这里，我们综述了近年来在免疫分析、生化分析和分子诊断方面离心微流控平台的最新进展。在免疫分析中，我们重点介绍酶联免疫吸附测定（ELISA）的应用；在生化分析中，我们介绍了血浆和血细胞分离的应用；在分子诊断中，我们强调了核酸扩增检测的应用。此外，我们还讨论了每个平台下方法的特点以及相应性能参数的增强，例如检测限、分离效率等。最后，我们讨论了现有应用中存在的局限性以及未来该领域可能取得的突破。
