Akbarmehr Amir, Peighambardoust Seyed Hadi, Soltanzadeh Maral, Jafari Seid Mahdi, Sarabandi Khashayar
Department of Food Science, College of Agriculture, University of Tabriz, Tabriz 5166616471, Iran.
Department of Food Science, College of Agriculture, University of Tabriz, Tabriz 5166616471, Iran.
Int J Biol Macromol. 2023 Apr 15;234:123678. doi: 10.1016/j.ijbiomac.2023.123678. Epub 2023 Feb 15.
Effects of hydrolyzed whey protein concentrate (WPC) and its combination with polysaccharides as wall material in spray-drying microencapsulation of Yerba mate extract (YME) have not been investigated yet. Therefore, it is hypothesized that the surface-active properties of WPC or WPC-hydrolysate may improve different properties of spray-dried microcapsules (such as physicochemical, structural, functional and morphological properties) compared to neat MD and GA. Thus, the objective of current study was to produce microcapsules loaded with YME by different carrier combinations. Effect of maltodextrin (MD), maltodextrin-gum Arabic (MD-GA), maltodextrin-whey protein concentrate (MD-WPC), and maltodextrin-hydrolyzed WPC (MD-HWPC) as encapsulating hydrocolloids was studied on physicochemical, functional, structural, antioxidant and morphological characteristics of the spray-dried YME. The type of carrier significantly affected spray dying yield. Enzymatic hydrolysis by improving the surface activity of WPC increased its efficiency as a carrier and produced particles with high production yield (about 68 %) and excellent physical, functional, hygroscopicity and flowability indices. Chemical structure characterization by FTIR indicated the placement of phenolic compounds of the extract in the carrier matrix. FE-SEM study showed that the microcapsules produced with polysaccharide-based carriers were completely wrinkled, whereas, the surface morphology of particles was improved when protein-based carriers were applied. Among the produced samples, the highest amount of TPC (3.26 mg GAE/mL), inhibition of DPPH (76.4 %), ABTS (88.1 %) and hydroxyl (78.1 %) free radicals were related to microencapsulated extract with MD-HWPC. The results of this research can be used to stabilize plant extracts and produce powders with appropriate physicochemical properties and biological activity.
水解乳清蛋白浓缩物（WPC）及其与多糖组合作为壁材在喷雾干燥微胶囊化马黛茶提取物（YME）中的作用尚未得到研究。因此，据推测，与纯麦芽糊精（MD）和阿拉伯胶（GA）相比，WPC或WPC水解产物的表面活性特性可能会改善喷雾干燥微胶囊的不同特性（如物理化学、结构、功能和形态特性）。因此，本研究的目的是通过不同的载体组合制备负载YME的微胶囊。研究了麦芽糊精（MD）、麦芽糊精-阿拉伯胶（MD-GA）、麦芽糊精-乳清蛋白浓缩物（MD-WPC）和麦芽糊精-水解WPC（MD-HWPC）作为包封水胶体对喷雾干燥YME的物理化学、功能、结构、抗氧化和形态特征的影响。载体类型对喷雾干燥产率有显著影响。通过提高WPC的表面活性进行酶水解提高了其作为载体的效率，并产生了高产率（约68%）且具有优异物理、功能、吸湿性和流动性指标的颗粒。傅里叶变换红外光谱（FTIR）进行的化学结构表征表明提取物中的酚类化合物存在于载体基质中。场发射扫描电子显微镜（FE-SEM）研究表明，用多糖基载体制备的微胶囊完全起皱，而应用蛋白基载体时颗粒的表面形态得到改善。在所制备的样品中，总酚含量（TPC）最高（3.26 mg GAE/mL）、对二苯基苦味酰基自由基（DPPH）的抑制率（76.4%）、对2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸阳离子自由基（ABTS）的抑制率（88.1%）和对羟基自由基的抑制率（78.1%）均与MD-HWPC微胶囊化提取物有关。本研究结果可用于稳定植物提取物并制备具有适当物理化学性质和生物活性的粉末。
