Mihalcea Liliana, Păcularu-Burada Bogdan, Milea Ștefania-Adelina, Aprodu Iuliana, Condurache Lazăr Nina-Nicoleta, Cucolea Elena Iulia, Dănilă George-Mădălin, Cîrciumaru Adrian, Nicoleta Stănciuc
Dunărea de Jos University of Galati, Faculty of Food Science and Engineering, Domnească Street 111, 800201, Galati, Romania.
Cromatec Plus SRL, Research Center for Instrumental Analysis SCIENT, Petre Ispirescu Street 1, Tâncăbești, Ilfov, 077176, Romania.
Curr Res Food Sci. 2023 Jan 23;6:100449. doi: 10.1016/j.crfs.2023.100449. eCollection 2023.
The supercritical fluids extraction (SFE) was used to extract the oleoresins from rosehip, followed by an in-depth phytochemical analysis and the development of two design-customized powders for different food and pharmaceutical applications. The SFE experiments allowed obtaining an oleoresins extraction yield of 11.85%. Two fractions were separated (S40 and S45), with significantly different phytochemical profile (p < 0.05), highlighting the efficiency of extraction of fatty acids in S40 extract, whereas the extraction of polyphenols, phytosterols, carotenoids and polyphenols was favored in S45 extract. The phytochemical profile revealed that the linoleic acid (C18:2) and α-linolenic acid (C18:3) represented approximatively 82% and 58% from the total fatty acid content in S40 and S45, respectively. α-Tocopherol and γ-tocopherol prevailed in both extract fractions, with a higher concentration in S45 (229.66 mg/g dry matter (DM) and 112.36 mg/g DM, respectively), whereas β-sitosterol was the major phytosterol in S45 fraction (118.75 mg/g DM). The S40 fraction was used to design two microencapsulated powders, by combining emulsification, complex coarcevation and freeze-drying. In order to develop new wall materials, with unique properties, the soy protein isolates were used for cross-linked reactions, by using an approach in one step (transglutaminase mediated) (coded as N) and two-steps (heat-induced and transglutaminase mediated) (coded as T). The N powder showed a better phytochemical content, leading to a higher antioxidant activity (5.27 mM Trolox equivalents/g DM), whereas for variant T, the bioactive were apparently doubled encapsulated.
采用超临界流体萃取（SFE）法从玫瑰果中提取油树脂，随后进行深入的植物化学分析，并开发两种针对不同食品和制药应用定制设计的粉末。SFE实验的油树脂提取率为11.85%。分离出两个馏分（S40和S45），其植物化学特征有显著差异（p < 0.05），突出了S40提取物中脂肪酸的提取效率，而S45提取物中多酚、植物甾醇、类胡萝卜素和多酚的提取效果更佳。植物化学特征表明，亚油酸（C18:2）和α-亚麻酸（C18:3）分别约占S40和S45总脂肪酸含量的82%和58%。α-生育酚和γ-生育酚在两个提取物馏分中均占主导地位，S45中的浓度更高（分别为229.66 mg/g干物质（DM）和112.36 mg/g DM），而β-谷甾醇是S45馏分中的主要植物甾醇（118.75 mg/g DM）。通过乳化、复合凝聚和冷冻干燥相结合的方法，使用S40馏分设计了两种微胶囊粉末。为了开发具有独特性能的新型壁材，采用一步法（转谷氨酰胺酶介导）（编码为N）和两步法（热诱导和转谷氨酰胺酶介导）（编码为T）对大豆分离蛋白进行交联反应。N粉末的植物化学成分含量更高，导致抗氧化活性更高（5.27 mM Trolox当量/g DM），而对于变体T，生物活性成分的包封率明显提高了一倍。
